中国现代医学杂志2017，Vol.27Issue(12)：1-8,8.DOI:10.3969/j.issn.1005-8982.2017.12.001
美洲大蠊提取物对人肝癌HepG2细胞的作用机制研究
Effect of Periplaneta Aamericana extracts on HepG2 cells*
摘要
Abstract
Objective To investigate the effect of extracts from Periplaneta Aamericana on in vitro prolife-ration, migration and VEGF protein expression of human hepatocellular carcinoma HepG2 cells. Methods Scratch assay was applied to measure the effect of extracts from Periplaneta americana on migration of HepG2 cells. Immunohistochemical staining and enzyme-linked immunosorbent assay (ELISA) were used to detect the effect of extracts from Periplaneta america on the expression of VEGF protein in the cells. Results MTT assay and Scratch assay showed that Periplaneta americana polypeptides, CⅡ-3 and skimmed cream could inhibit the proliferation and migration of HepG2 cells in a time- and dose-dependent manner. Immunohistochemical staining and ELISA indicated that Periplaneta americana polypeptides, CⅡ-3 and skimmed cream could down regulate the expression of VEGF protein in the cells. Conclusions Periplaneta americana polypeptides, CⅡ-3 and skimmed cream can inhibit the in vitro proliferation and migration of HepG2 cells in vitro in a time-and dose-dependent way, and reduce the expression of VEGF protein in the cells; and the effect of Periplaneta americana extracts is better than that of CⅡ-3 and skimmed cream.关键词
美洲大蠊多肽/HepG2细胞/增殖和迁移/VEGF表达Key words
Periplaneta americana polypeptide/HepG2 cells/proliferation and migration/VEGF分类
医药卫生引用本文复制引用
张蕊,袁发璐,李婷,彭芳..美洲大蠊提取物对人肝癌HepG2细胞的作用机制研究[J].中国现代医学杂志,2017,27(12):1-8,8.基金项目
国家自然科学基金(No:81560600) （No:81560600）
