中国现代医学杂志2017，Vol.27Issue(12)：15-20,6.DOI:10.3969/j.issn.1005-8982.2017.12.003
GPP34激活结肠癌细胞Wnt信号通路促进癌细胞增殖的研究
GPP34 promotes colon cancer cell proliferation through activation of Wnt signaling pathway*
摘要
Abstract
Objective To investigate the mechanism of high-expression of Golgi-associated protein of 34 (GPP34) gene in activation of Wnt signaling pathway to promote colon cancer cell proliferation. Methods Colon cancer cell lines were divided into four groups: control group, siRNA transfection group, the group of added inhibitor of Akt/Protein Kinase B (Tricinbine group), and the group of added inhibitor of GSK-3β(TWS119 group). The cells were cultured respectively. The silencing effect of GPP34 gene was detected by RT-PCR. The proliferation and apoptosis of the cancer cells in all groups were detected by MTT and flow cytometry respectively. Topflash reporter gene activity assay was used to detect the activity of Wnt signaling pathway in the cancer cells of each group. The expressions of GPP34, β-catenin and pS9-GSK-3β proteins in the cancer cells of all groups were detected by Western blot. Results The expression level of GPP34 mRNA in the transfection group was significantly lower than that in the control group (P<0.05). Compared to the control group, the growth inhibition rate and the apoptosis rate were significantly increased (P<0.05), and the activity of Wnt signaling pathway was significantly inhibited in each experimental group (P<0.05). However, the growth inhibition rate, the apoptosis rate and the activity of Wnt signaling pathway showed no significant differences among the experimental groups (P>0.05). Compared to the control group, GPP34 protein expression significantly decreased in the siRNA transfection group (P<0.05), while that in the other two experimental groups had no significant difference (P>0.05); β-catenin and pS9-GSK-3β protein expressions were signifi-cantly decreased in the three experimental groups (P<0.05). Conclusions High-expression of GPP34 can acti-vate the classical Wnt signaling pathway to promote proliferation and inhibit apoptosis of SW620 colon cancer cells.关键词
人结肠癌细胞/高尔基相关蛋白34/Wnt信号通路/增殖/糖原合酶激酶-3β/β-cateninKey words
human colorectal cancer cell line/GPP34/Wnt signaling pathway/proliferation/glycogen synthase kinase-3β/β-catenin分类
医药卫生引用本文复制引用
汪明振,邱成志,余外市,王春晓,郭延塔..GPP34激活结肠癌细胞Wnt信号通路促进癌细胞增殖的研究[J].中国现代医学杂志,2017,27(12):15-20,6.基金项目
福建省自然科学基金(No:2015J01438) （No:2015J01438）
福建医科大学校教授基金(No:JS14028) （No:JS14028）
