中国现代医学杂志2017，Vol.27Issue(12)：25-29,5.DOI:10.3969/j.issn.1005-8982.2017.12.005
脂肪移植治疗DNCB诱导ACD模型小鼠的实验研究
Research on fat transplantation treatment for DNCB-sensitized allergic contact dermatitis of mouse model*
摘要
Abstract
Objective To explore whether fat transplantation can improve the symptoms of the mouse model of allergic contact dermatitis (ACD) induced by dinitrochlorobenzene (DNCB) as well as its possible mecha-nism. Methods ACD mouse models were randomly divided into group A (fat transplantation group), group B (pseudo surgery group), group C (medication group) and group D (spontaneously-recovered model group). The HE-stained skin tissues of each group were observed under light microscope. The expression levels of IL-10, IL-17 and IFN-γ mRNAs in the skin lesions were detected by qRT-PCR. Results In the groups A and C, the horny layers became thinner, and the thickness of epidermis recovered to normal while there were no overt changes in the groups B and D. The expression levels of IL-10 and IL-17 mRNAs in the skin tissues of the groups A and C were clearly lower than those of the group D, the level of IFN-γ mRNA was higher than that of the group D (P<0.05); however, there was no significant changes in the IL-10, IL-17 or IFN-γmRNA level between the groups B and D. Conclusions Fat transplantation can decrease the expression levels of IL-10 and IL-17 in skin lesions, promote the production of IFN-γ, reduce the symptoms of skin lesions and promote the recovery of skin lesions in the mouse model.关键词
脂肪移植/变应性接触性皮炎/辅助性T细胞1/辅助性T细胞2/Treg/Th17Key words
fat transplantation/allergic contact dermatitis/Th1/Th2/Treg/Th17分类
医药卫生引用本文复制引用
旷翎,贾晓敏,冯浩,伍博,徐晓芃..脂肪移植治疗DNCB诱导ACD模型小鼠的实验研究[J].中国现代医学杂志,2017,27(12):25-29,5.基金项目
湖南省教育厅优秀青年项目(No:15B139) （No:15B139）
湖南省卫生计生委项目(No:C2017035) （No:C2017035）
