中国现代医学杂志2017，Vol.27Issue(12)：30-34,5.DOI:10.3969/j.issn.1005-8982.2017.12.006
芪参益气滴丸对大鼠心肌缺血再灌注损伤的作用机制研究
Protective effect of Qishenyiqi Dripping Pills on rats with myocardial ischemia-reperfusion injury
陈君 1李蕾 2陈国华 3刘传亮 2李文通1
作者信息
- 1. 潍坊医学院 研究生院,山东 潍坊 261042
- 2. 山东省潍坊市人民医院 保健三科,山东 潍坊 261041
- 3. 山东省潍坊市人民医院 健康管理中心,山东 潍坊 261041
- 折叠
摘要
Abstract
Objective To investigate the effect of Qishenyiqi Dripping Pills on urothelial carcinoma antigen 1 (UCA1) LncRNA, apoptosis and apoptosis related gene expression of myocardial cells in rats with myocardial ischemia reperfusion. Methods Ischemia reperfusion injury model of SD rats was established by ligating left anterior descending branch of coronary artery for 40 min followed by reperfusion for 120 min. Thirty-six rats were randomly divided into sham operation group (only puncture wihout ligation), ischemia-reperfusion group (IR group) and Qishenyiqi intervention group (QS+IR group). After operation, the apoptosis index was detected by TUNEL method; fluorescence quantitative PCR technique and Western blot were used to detect the expression levels of UCA1 and p27 protein respectively. Results The number of apoptosis in the QS+IR group was significantly smaller than that in the IR group (P<0.05). The overall level of UCA1 expression significantly increased and p27 protein markedly decreased in the QS+IR group compared with the IR group (P<0.05); and the expression of UCA1 was negatively correlated with the expression of p27 protein. Conclusions Qishenyiqi Dripping Pills can reduce myocardial apoptosis rate in rats with myocardial ischemia and reperfusion, which may protect the injured myocardial cells by increasing the level of UCA1 and reducing the expression of p27 protein.关键词
芪参益气滴丸/心肌缺血再灌注/细胞凋亡/尿路上皮癌胚抗原1/p27蛋白Key words
Qishenyiqi Dropping Pill/myocardial ischemia reperfusion/apoptosis/urothelial carcinoma antigen 1/p27 protein分类
医药卫生引用本文复制引用
陈君,李蕾,陈国华,刘传亮,李文通..芪参益气滴丸对大鼠心肌缺血再灌注损伤的作用机制研究[J].中国现代医学杂志,2017,27(12):30-34,5.
