中国现代医学杂志2017，Vol.27Issue(12)：35-39,5.DOI:10.3969/j.issn.1005-8982.2017.12.007
长链非编码RNA PRNCR1对胃癌细胞增殖、侵袭及转移的研究
Long non-coding RNA PRNCR1 promotes proliferation,
invasion and migration of gastric cancer cells
蔡毅1
作者信息
- 1. 南华大学附属第一医院 急诊内科,湖南 衡阳 421001
- 折叠
摘要
Abstract
Objective To observe the expressions of long non-coding RNA PRNCR1 in gastric cancer MGC-803 cells and normal gastric mucosa epithelial GES-1 cells, and to study the effect of PRNCR1 on the proliferation, migration and invasion of gastric cancer cells by silencing the expression of PRNCR1. Methods siRNA fragments (PRNCR1-siRNA) and control fragments (PRNCR1-NC) were designed, synthesized and transfected to gastric cancer cells. The expression of PRNCR1 was detected by qRT-PCR. Cell proliferation was detected by MTT. Migration and invasion of gastric cancer cells were detected by Transwell assay. Results The expression of PRNCR1 in the MGC-803 cells was higher than that in the GES-1 cells. The expression of PRNCR1 was reduced by PRNCR1-siRNA in the MGC-803 cells. The proliferation, invasion and migration of the MGC-803 cells decreased after transfection of PRNCR1-siRNA. Conclusions The expression of PRNCR1 can be down-regulated by PRNCR1-siRNA, and PRNCR1-siRNA could inhibit the proliferation, invasion and migration of gastric cancer cells, which provides a theoretical foundation for gene therapy of gastric cancer targeting at PRNCR1 gene.关键词
长链非编码RNA/前列腺癌非编码RNA1/增殖/侵袭/转移/胃癌Key words
long non-coding RNA/PRNCR1/proliferation/invasion/migration/gastric cancer分类
医药卫生引用本文复制引用
蔡毅..长链非编码RNA PRNCR1对胃癌细胞增殖、侵袭及转移的研究[J].中国现代医学杂志,2017,27(12):35-39,5.
