中国现代医学杂志2017，Vol.27Issue(12)：46-49,4.DOI:10.3969/j.issn.1005-8982.2017.12.009
膀胱尿路上皮癌中PTEN表达与其基因甲基化的关系
Correlation between expression of PTEN and its promoter methylation in bladder urothelial cell carcinoma*
摘要
Abstract
Objective To investigate the relationship between expression of phosphate and tension homology deleted on chromsome ten (PTEN) gene and its promoter methylation in bladder urothelial cell carcinoma (BUCC). Methods The protein expression and promoter methylation of PTEN gene were detected in 41 cases of BUCC and 18 normal bladder tissues using Western blot and Methylation Specific PCR (MSP) method respectively, and their correlation was analyzed. Results PTEN protein expression in the BUCC was lower than that in the normal bladder tissues (P<0.05). And the promoter methylation rate of PTEN gene in the BUCC (53.66%, 22/41) was significantly higher than that (0.00%, 0/18) in the normal bladder tissues (P<0.05). The expression level of PTEN protein in the BUCC of the methylation negative group was obviously lower than that of the methylation positive group (P<0.05). Conclusions The low expression of PTEN protein in BUCC is closely related with the high methylation of PTEN gene promoter.关键词
膀胱尿路上皮癌/磷酸酶-张力蛋白基因/DNA甲基化Key words
bladder urothelial cell carcinoma/phosphate and tension homology deleted on chromsome ten/DNA methylation分类
医药卫生引用本文复制引用
尹永华,程文杰,延敏博..膀胱尿路上皮癌中PTEN表达与其基因甲基化的关系[J].中国现代医学杂志,2017,27(12):46-49,4.基金项目
广东省深圳市科技计划项目(No:JCYJ20140414160300578) （No:JCYJ20140414160300578）
