中国药理学通报2017，Vol.33Issue(6)：767-771,5.DOI:10.3969/j.issn.1001-1978.2017.06.007
GDF11在棕榈酸诱导骨骼肌细胞胰岛素抵抗中的作用研究
Role of GDF11 in skeletal muscle cell insulin resistance induced by palmitate
摘要
Abstract
Aim To investigate the role of GDF11 in palmitate induced skeletal muscle insulin resistance.Methods The C2C12 cells were sorted into control group, GDF11 intervention group, palmitate group and GDF11 combined with palmitate group.Cell viability was measured by CCK-8, and the glucose uptake was determined by 2NBDG.The mRNA level of myotube marker genes(desmin,myogenin), insulin mediate glucose uptake related genes(GLUT-4,IRS-1) and PGC-1α were tested by RT-PCR.The protein expression of PGC-1α was detected by western blot.Results GDF11 had little effect on cell viability of skeletal muscle cells.Compared with control group, the glucose uptake and the expression of GLUT-4,IRS-1,PGC-1α were significantly decreased by palmitate intervention.Compared with palmitate group, the glucose uptake and the expression of GLUT-4,IRS-1,PGC-1α were not significantly changed by GDF11.Conclusion Palmitate can induce skeletal muscle cell insulin resistance, but GDF11 may not significantly improve the skeletal muscle cell insulin resistance.关键词
GDF11/骨骼肌细胞/胰岛素抵抗/PGC-1α/棕榈酸/葡萄糖摄取Key words
GDF11/skeletal muscle cell/insulin resistance/PGC-1α/palmitate/glucose uptake分类
医药卫生引用本文复制引用
敬媛媛,吴凡,李蓉..GDF11在棕榈酸诱导骨骼肌细胞胰岛素抵抗中的作用研究[J].中国药理学通报,2017,33(6):767-771,5.基金项目
国家自然科学基金资助项目(No 81200588) （No 81200588）
国家临床重点专科建设项目(No 2011-170) （No 2011-170）
