中国药理学通报2017，Vol.33Issue(6)：813-818,6.DOI:10.3969/j.issn.1001-1978.2017.06.014
两株红树林根际土壤放线菌的鉴定和抗菌活性研究
Identification and antimicrobial activity research on two actinomycetes isolated from mangrove rhizosphere soils
摘要
Abstract
Aim To identify and study antimicrobial acticity of actinomycetes isolated from mangrove rhizosphere soils in Beilun Estuary, Guangxi,China.Methods The typical morphological characteristics of two actinomycetes were observed.To determine the phylogenetic position of the isolates, 16S rRNA gene sequences were obtained by PCR amplification and direct sequencing, and then compared with the gene database, the phylogenetic tree was constructed using the neighboring method.Crude extracts were obtained from fermentation broth extracted by ethyl acetate and mycelia extracted by acetone, respectively.The antibacterial and antifungal activities of the crude extracts were evaluated using the disk diffusion method.Results Significant broad-spectrum antibacterial activities of two actinomycetes were isolated from mangrove rhizosphere soils in Beilun Estuary.The highest similarity of 16S rRNA gene sequence between strain B200 and the validly described species Agromyces subbeticus DSM16689T, between strain B205 and the validly described species Micromonospora rosaria DSM803T was 98.01% and 99.73%, respectively, which indicated that the strain B200 was Agromyces potential new taxa, and B205 was identified as Micromonospora rosaria DSM 803T variants.Conclusions Two actinomycetes isolated from mangrove rhizosphere soils in Beilun Estuary,Guangxi,and its fermentation products show significant antibacterial activity.Mangrove rhizosphere soils are rich in culturable medicinal actinobacteria, which could be a potential source for the discovery of new antibiotics, and deserves further research.关键词
北仑河口/红树林/放线菌/抗菌活性/耐药菌/16SrRNAKey words
Beilun Estuary/mangrove/actinomycetes/antibiotic activity/drug resistant bacteria/16S rRNA分类
医药卫生引用本文复制引用
吴越,李小俊,陈建宏,蒋莲秀,何玉林,韦晗宁,孙承航,黄大林..两株红树林根际土壤放线菌的鉴定和抗菌活性研究[J].中国药理学通报,2017,33(6):813-818,6.基金项目
国家自然科学基金资助项目(No 81460537) （No 81460537）
广西省自然科学基金资助项目(No 2014GXNSFAA118196) （No 2014GXNSFAA118196）
