中国药理学通报2017，Vol.33Issue(6)：844-848,5.DOI:10.3969/j.issn.1001-1978.2017.06.019
二甲双胍通过减少STAT3磷酸化缓解大鼠骨癌痛
Metformin attenuates bone cancer pain by inhibiting expression of p-STAT3
摘要
Abstract
Aim To investigate the effects of metformin on phospho-signal transducers and activators of transcription 3(p-STAT3) expression in the spinal dorsal horn in rats with bone cancer pain(BCP).Methods Healthy female SD rats weighing 200~220 g were randomly divided into four groups(n=12 each): Sham+NS, Sham+Metformin, BCP+NS, BCP+Metformin groups.Normal saline or metformin(200 mg·kg-1) was given intraperitoneally once a day at 7~14 day after surgery in four groups.Mechanical withdrawal threshold(MWT) was measured on-1, 3, 5, 7, 10, 12, 14 day after BCP.The L4-6 segments of spinal cords were used to detect the expression of p-STAT3 by Western blot and immunohistochemical staining technique after the behavioral test was completed on day 14.Results Intraperitoneal injection of metformin reversed hyperalgesia and suppressed the expression of p-STAT3 in BCP rats.Conclusion Metformin can attenuate BCP by inhibiting the expression of p-STAT3 in the spinal dorsal horn.关键词
骨肿瘤/疼痛/二甲双胍/信号转导子与转录激活子3/磷酸化/脊髓背角/大鼠Key words
bone neoplasms/pain/metformin/signal transducers and activators of transcription 3/phosphorylation/spinal dorsal horn/rat分类
医药卫生引用本文复制引用
葛安琪,殷红,李登峰,张辉,赵伟,颜明,苗蓓..二甲双胍通过减少STAT3磷酸化缓解大鼠骨癌痛[J].中国药理学通报,2017,33(6):844-848,5.基金项目
国家自然科学基金资助项目(No 81200861) （No 81200861）
