中国药理学通报2017，Vol.33Issue(6)：869-873,5.DOI:10.3969/j.issn.1001-1978.2017.06.024
异丙酚对宫内窘迫胎鼠的神经保护作用
Neuroprotective effect of propofol on fetal ratbrain in intrauterine ischemia/reperfusion injury
摘要
Abstract
Aim To observe the neuroprotective effect of different doses of propofol on ischemic fetal rat brain.Methods Eighteen healthy pregnant SD rats were randomly allocated into the following six groups with three rats in each.Group S: sham operation group, Group IR: ischemia/reperfusion group, Group P1~P3: different doses of propofol groups, Group B: bicuculline group.In group S and group IR, 1 ml saline solution was administered via caudal vein.In group P1~P3, 10, 30, 50 mg·kg-1 of propofol was administered via caudal vein respectively.In group B, when 50 mg·kg-1 propfol was administered via caudal vein, 5 mg·kg-1 bicuculline was injected intraperitoneally at the same time.Bilateral uterine ovarian arteries were clamped for 11 mins to make intrauterine distress model of the fetal rats.The brains of fetal rats were removed after 3 days of reperfusion.Brain sections(5 μm thick) were mounted and stained with Hematoxylin and eosin(HE).The profile of the hippocampus CA1 was evaluated under a light microscope and neuronal Lesion-index(LI) was calculated.MDA content of fetal rat brain was detected by thiobarbituric acid reaction method to determine the lipid peroxidation degree of brain.Results LI was (7.2±0.9) and MDA was (3.86±0.20) μmol·g-1 in group S.LI was 71.9±2.8 and the content of MDA was (9.10±0.45) μmol·g-1 in group IR, which increased significantly compared with those in group S(P<0.01).LI was (40.8±2.6), (21.4±1.4), (20.1±1.3) and the content of MDA was (7.32±0.41), (5.65±0.27), (5.44±0.28) μmol·g-1 in propofol groups, which decreased significantly compared with those in group IR(P<0.05).LI and the content of MDA was (51.2±2.3), (7.54±0.31) μmol·g-1 in group B,respectively, reversing partly the neuroprotevtive effect of propofl.Conclusion Propofol could protect the neurons in hippocampus CA1 region of fetal rat against intrauterine distress by reducing the concentration of MDA in the brain.关键词
异丙酚/胎鼠宫内窘迫/缺血/再灌注损伤/神经元损伤指数/丙二醛/荷包牡丹碱Key words
propofol/fetal rat intrauterine distress/ischemia/reperfusion injury/Lesion-index of neuron/MDA/bicuculline分类
医药卫生引用本文复制引用
蔡劲松,冯帅,戚翔,梁治,徐雪..异丙酚对宫内窘迫胎鼠的神经保护作用[J].中国药理学通报,2017,33(6):869-873,5.基金项目
河北省科技厅计划项目(No 132777129 （）
