中国医学装备2017，Vol.14Issue(6)：156-160,5.DOI:10.3969/J.ISSN.1672-8270.2017.06.043
X射线及UVB对人表皮细胞细胞增殖及褪黑素受体表达的影响
Effects of X-ray and UVB for proliferation and the expression of melatonin receptor of human epidermal cells
摘要
Abstract
Objective:To explore the effects of X-rays and ultraviolet B (UVB) for the proliferation of human epidermal cells and the expression of melatonin receptor in human epidermal cells. This study can provide guidance about radiation effect and how to protect the public who may suffered higher ionization and non-ionizing radiation in high altitude region. Methods: Human epidermal cells (Hacat) and human melanoma cell lines A875 were irradiated with X-ray of different doses (0, 0.5, 2 and 5 Gy) and UVB of different doses (20, 50, 100 and 200 mJ/cm2), respectively. The change of cell proliferative capacity was detected by using methyl thiazolyl tetrazolium (MTT) and cloning efficiency experiment, and the change about expression of melatonin receptor (MTNR-1B) was detected by using western blot.Results: Cell proliferative capacity of Hacat and A875 were decreased in various degrees when they exposed in X-ray of different doses (0.5, 2 and 5 Gy) and ultraviolet B of different dose (20, 50 mJ/cm2). For Hacat cells, the cloning efficiencies of them in observation group after were irradiated by using X-ray of 3 kinds of doses were 71.2%, 36.7% and 16.3% of that in control group, and the differences between the two group were statistically significant (F=249.96,P<0.05), respectively. While for A875 cells, the cloning efficiencies of them in observation group were 64.7%, 49.5% and 31.6% of that in control group at the same 3 kinds of doses, and the differences between the two group also were statistically significant (F=147.29,P<0.05), respectively. On the other hand, after Hacat cells were radiated by using UVB of different doses (20 and 50 mJ/cm2), the cloning efficiencies of them in observation group were 40.74% and 12.96% of that in control group, respectively. And for A875 cell, they were 16.33% and 6.12% of that in control group at the same condition of dose, respectively. In addition, after Hacat cells were radiated by using UVB of 50 mJ/cm2, 100 mJ/cm2 and 200 mJ/cm2, respectively, the results revealed that expression of MTNR1B was significantly increased with the enhancing of dose, while the X-ray of 3 kinds of doses were no significant effect for the expression of MTNR1B.Conclusion: Both of X-ray irradiation and UVB irradiation can cause the reductions of the capacities of proliferation and survival, and the inhibition of cell proliferation might be relative with the change of expression of melatonin receptor after UVB radiates cell.关键词
X射线/紫外线B(UVB)/细胞增殖/褪黑素受体/蛋白印迹法Key words
X-ray/Ultraviolet-b(UVB)/Cell proliferation/Melatonin receptor/Western blot分类
医药卫生引用本文复制引用
高玲,李至荟,封江彬,陆雪,蔡恬静,李爽,赵骅,田雪蕾,刘青杰..X射线及UVB对人表皮细胞细胞增殖及褪黑素受体表达的影响[J].中国医学装备,2017,14(6):156-160,5.基金项目
国家自然科学基金面上项目(31570852)"STAT3调控caveolin-1介导的抗早衰在肿瘤辐射抗性中的作用及机制研究" （31570852）
北京市自然科学基金面上项目(7162137)"UVB紫外线致黑素细胞早衰及对黑色素合成的调节机制研究" （7162137）
中国疾病预防控制中心青年科研基金(2015A201)"利用负载lewis细胞的小鼠模型评价低剂量辐射对机体抗肿瘤免疫功能的影响" （2015A201）
