中国药业2017，Vol.26Issue(10)：18-20,3.DOI:10.3969/j.issn.1006-4931.2017.10.005
别嘌醇联合5-氟尿嘧啶对肝癌HepG2细胞增殖及凋亡的影响
Influence of Allopurinol Combined with 5-Fluorouracil on Proliferation and Apoptosis of Liver Cancer HepG2 Cells
侯小梅 1冯安勇 2严海元 1冷崇姣1
作者信息
- 1. 重庆市永川食品药品检验所,重庆 402160
- 2. 重庆市计量质量检测研究院第三分院,重庆 402160
- 折叠
摘要
Abstract
Objective To study the influence of Allopurinol combined with 5-Fluorouracil on the proliferation and apoptosis of liver cancer HepG2 cells. Methods The HepG2 cells were treated by different concentrations and combinations of Allopwrind,5-Fluo-rouracil. CCK-8 was used to measure HepG2 cells proliferation,and flow cytometry was used to examine the apoptosis of HepG2 cells. Results The inhibition of HepG2 cells apoptosis reacted by Allopurinol was weak,in combination inhibited the proliferation of HepG2 cells in a time (24,48,72 h)and concentration dependent (1-3 μg/L)manner. Allopurinol combined with 5-Fluorouracil had stronger inhibitory effect than that was used alone. The apoptosis rate of HepG2 cells in the combination treatment group was 39. 17%,which was significantly higher than 30. 86% in the 5-Fluorouracil group(P < 0. 05). Conclusion Allopurinol combined with 5-Fluorouracil has enhanced effect on the proliferation and apoptosis of HepG2 cells.关键词
5-氟尿嘧啶/别嘌醇/HepG2细胞/增殖/凋亡Key words
5-Fluorouracil/Allopurinol/HepG2 cells/proliferation/apoptosis分类
医药卫生引用本文复制引用
侯小梅,冯安勇,严海元,冷崇姣..别嘌醇联合5-氟尿嘧啶对肝癌HepG2细胞增殖及凋亡的影响[J].中国药业,2017,26(10):18-20,3.
