中国中医药信息杂志2017，Vol.24Issue(7)：66-71,6.DOI:10.3969/j.issn.1005-5304.2017.07.016
响应面法优化复合酶提取枸杞子总黄酮的工艺研究
Optimization of Compound Enzyme Extraction Technology of Total Flavonoids from Lycii Fructus by Response Surface Method
摘要
Abstract
Objective To optimize the extraction technology of total flavonoids from Lycii Fructus by response surface methodology (RSM).Methods On the basis of single-factor tests, enzyme concentration, enzymolysis time, and enzyme solution temperature were selected as influencing factors during extraction to conduct three-factor three-level center combination design. The effects of three factors on the yield of total flavonoids from Lycii Fructus were analyzed by RSM.Results The optimum conditions for extraction technology of total flavonoids from Lycii Fructus were enzyme concentration 0.30%, enzymolysis time 1.0 h, enzyme solution temperature 60℃. The predicted extraction yield of total flavonoids was 0.9492%.ConclusionUsing RSM to optimize the extraction technology of total flavonoids from Lycii Fructus is stable, feasible, and simple, which can provide references for further study and application.关键词
响应面法/工艺优化/复合酶提取/枸杞子总黄酮Key words
response surface method/technology optimization/compound enzyme extraction/total flavonoids from Lycii Fructus分类
医药卫生引用本文复制引用
高建德,朱晓玉,刘雄,陈晖,宋开蓉,师婷婷..响应面法优化复合酶提取枸杞子总黄酮的工艺研究[J].中国中医药信息杂志,2017,24(7):66-71,6.基金项目
甘肃省科技攻关项目(GYC13-05) （GYC13-05）
甘肃省高等学校科学研究项目(2015B-073) （2015B-073）
