中成药2017，Vol.39Issue(6)：1187-1190,4.DOI:10.3969/j.issn.1001-1528.2017.06.016
HPLC法同时测定当飞利肝宁胶囊中7种成分
Simultaneous determination of seven constituents in Dangfei Liganning Capsules by HPLC
龙玉堂 1陈浩 1刘晶1
作者信息
- 1. 南华大学附属第一医院,湖南衡阳421001
- 折叠
摘要
Abstract
AIM To establish an HPLC method for the simultaneous content determination of swertiamarin,gentiopicrin,swertianolin,taxifolin,silychristin,silydianin and silybin in Dangfei Liganning Capsules (Silybi Fructus and Swertiae Herba).METHODS The analysis of 75％ methanol extract of this drug was performed on a 30 ℃ thermostatic Kromasil C18 column (4.6 mm × 250 mm,5.0 μm),with the mobile phase comprising of methanol-0.1％ formic acid flowing at 0.9 mL/min in a gradient elution manner,and the detection wavelengths were set at 254 nm and 288 nm.RESULTS Seven constituents showed good linear relationships within their own ranges (r ＞ 0.999 0),whose average recoveries were 96.78％-100.45％ with the RSDs of less than 2.0％.CONCLUSION This accurate and reproducible method can be used for the rapid quality control of Dangfei Liganning Capsules.关键词
当飞利肝宁胶囊/化学成分/HPLCKey words
Dangfei Liganning Capsules/chemical constituents/HPLC分类
医药卫生引用本文复制引用
龙玉堂,陈浩,刘晶..HPLC法同时测定当飞利肝宁胶囊中7种成分[J].中成药,2017,39(6):1187-1190,4.
