广东海洋大学学报2017，Vol.37Issue(3)：8-13,6.DOI:10.3969/j.issn.1673-9159.2017.03.002
墨吉明对虾c型溶菌酶基因的克隆及表达
Cloning and Expression of Gene of C-type Lysozyme from Fenneropenaeus merguiensis
摘要
Abstract
The gene of a c-type lysozyme, namely Fm-lyz, from Fenneropenaeus merguiensis was cloned and identified. The full-length cDNA of Fm-Lyz is 770 bp with 5' UTR of 71 bp, 3' UTR of 222 bp and an open reading frame(ORF) of 477 bp, which encodes a putative protein of 158 amino acids including a LYZ1 domain and a signal peptide with 18 amino acids. Multiple alignment analysis shows that Fm-Lyz shares the highest amino acid identity with Penaeus monodon. Phylogenetic analysis shows that Fm-Lyz is clustered into c-type lysozyme group of shrimp. qPCR analysis shows that Fm-Lyz is constitutively expressed in all the examined tissues with the highest expression in hemolymph. Upon Vibrio parahaemolyticus and Micrococcus lysodeikticus challenge, the expression of Fm-Lyz is significantly up-regulated compared with the control group (PBS group), and the time points of the highest expression is 24 h and 12 h, respectively, which suggests that Fm-Lyz may involve in anti-bacterial host defense.关键词
墨吉明对虾/c型溶菌酶/基因/克隆/表达Key words
Fenneropenaeus merguiensis/c-type lysozyme/gene/clone/expression分类
生物科学引用本文复制引用
史黎黎,周婷婷,李逸群,曾坤辉,陈兆明,王成桂,章双..墨吉明对虾c型溶菌酶基因的克隆及表达[J].广东海洋大学学报,2017,37(3):8-13,6.基金项目
广东省自然科学基金-博士启动项目(2016A030310334),广东省自然科学基金-自由申请项目(2016A030313757),广东海洋大学优秀青年骨干教师特别资助计划项目(HDYQ2017003,HDYQ2015004) （2016A030310334）
