广东海洋大学学报2017，Vol.37Issue(3)：38-42,5.DOI:10.3969/j.issn.1673-9159.2017.03.006
鰤鱼诺卡氏菌与绛红色诺卡氏菌的融合菌研究
Study on Protoplasts fusion of Nocardia seriolae and N. purpurea
摘要
Abstract
Nocardia seriolae, the main pathogen of fish nocardiosis, and N. purpurea, a non-toxic Nocardia sp. isolated from soil, were selected as parent strains for protoplast fusion.The fusion strains were identified by colony morphology, Gram staining and 16s rRNA sequencing. Then the virulence test and immune protective effects of the fusion strains were evaluated by the zebrafish model.The results showed that the fusant was Gram-positive nocardia bacteria, and its colony morphology was different from the parent strains. The virulence of fusant decreased 70% compared with wild strains N. seriolae ZJ0503 and the immune protection rate of fusant is about 50%.关键词
鰤鱼诺卡氏菌/绛红色诺卡氏菌/原生质体融合/毒力/免疫保护Key words
Nocardia seriolae/Nocardia purpurea/protoplast fusion/virulence/immune protection分类
农业科技引用本文复制引用
陈建林,严聪羚,张红莲,苏泽杰,夏立群..鰤鱼诺卡氏菌与绛红色诺卡氏菌的融合菌研究[J].广东海洋大学学报,2017,37(3):38-42,5.基金项目
广东省科技发展专项资金项目(2016A050502061),广东省自然科学基金(2014A030313602),深圳大鹏新区产业发展专项资金项目(KY20160207),广东省攀登计划项目(pdjh2016b0234) （2016A050502061）
