浙江医学2017，Vol.39Issue(9)：703-707,5.DOI:10.12056/j.issn.1006-2785.2017.39.9.2016-1908
大肠癌组织与癌旁正常组织基因差异表达图谱及信号通路研究
Differentially expressed genes in colorectal cancer tissues and adjacent normal tissues
摘要
Abstract
Objective To screen the differentially expressed genes in human colorectal cancer (CRC) tissue.Methods NimbleGen gene expression array information were used to detect the gene expressions of CRC tissue paired with normal colorectal tissue.The microarray findings were confirmed by real-time quantitative reverse transcriptase-polymerase chain reaction (RT-PCR).The results were analyzed by bioinformatic technique.Results Compared with normal tissues,a total of 5042genes with more than 2-fold differential expression were detected in CRC tissues,of which 3399 genes were up-regulated and1643 genes were down-regulated.These genes involved multiple signaling pathways.Conclusion The results may provide experimental basis to seek for molecular markers and to investigate the molecular mechanism of colorectal cancer.关键词
大肠癌/基因芯片/基因表达谱/生物信息学Key words
Colorectal cancer/Gene chips/Gene expression profiles/Bioinformatics引用本文复制引用
洪朝金,卢丽琴,郭勇,钦志泉..大肠癌组织与癌旁正常组织基因差异表达图谱及信号通路研究[J].浙江医学,2017,39(9):703-707,5.基金项目
浙江省医学会临床科研基金项目(2015ZYC-B1) （2015ZYC-B1）
浙江省中医药科技计划重大项目(2007ZA007) （2007ZA007）
