作物学报2017，Vol.43Issue(6)：789-794,6.DOI:10.3724/SP.J.1006.2017.00789
水稻组织特异型人工合成启动子的设计、构建及功能鉴定
Designing, Construction and Functional Characterization of Tissue-specific Synthetic Promoter in Rice
摘要
Abstract
As an important part of synthetic biology, synthetic promoter has gradually become a hotspot in current biology. Rice is one of the most important food crops in the world and a model plant for functional genomic research in cereals. The purpose of the present study was to design and construct tissue-specific synthetic promoters in rice. We selected several tissue-specific cis-elements based on previous reports and assembled them in different patterns. The combinations were respectively fused to Mini 35S promoter. GUS assays of the transgenic plants indicated that our work successfully created tissue-specific synthetic promoter with the above approach and also revealed that the assembly patterns of cis-elements are critical for the expression pat-terns and expression efficiencies of synthetic promoters. Our study provides useful information and a practical basis for the design ideas and construction approaches of synthetic promoters in plants.关键词
水稻/合成启动子/GUS报告基因/顺式调控元件Key words
Rice/Synthetic promoter/GUS reporter gene/cis-element引用本文复制引用
王睿,朱梦琳,高方远,任鄄胜,陆贤军,任光俊,林拥军..水稻组织特异型人工合成启动子的设计、构建及功能鉴定[J].作物学报,2017,43(6):789-794,6.基金项目
本研究由国家转基因生物新品种培育重大专项(2014ZX08001001)和国家高技术研究发展计划(863 计划)项目资助.This study was supported by the National Major Project for Developing New GM Crops (2014ZX08001001) and the National High Technol-ogy Research and Development Program of China (863 Program). （2014ZX08001001）
