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水稻重测序核心种质资源的稻瘟病抗性鉴定与评价

李旭升 徐建龙 向小娇 申聪聪 杨隆维 陈凯 王小文 邱先进 朱小源 邢丹英

作物学报2017,Vol.43Issue(6):795-810,16.
作物学报2017,Vol.43Issue(6):795-810,16.DOI:10.3724/SP.J.1006.2017.00795

水稻重测序核心种质资源的稻瘟病抗性鉴定与评价

Identification and Evaluation of Blast Resistance for Resequenced Rice Core Collections

李旭升 1徐建龙 2向小娇 3申聪聪 2杨隆维 2陈凯 1王小文 2邱先进 3朱小源 1邢丹英1

作者信息

  • 1. 长江大学主要粮食作物产业化湖北省协同创新中心, 湖北荆州 434025
  • 2. 中国农业科学院作物科学研究所, 北京 100081
  • 3. 中国农业科学院深圳农业基因研究所, 广东深圳 518210
  • 折叠

摘要

Abstract

Rice blast is one of the key factors that restrict rice yield, and screening blast resistance resources is a basis work for mining blast resistance genes and breeding resistant varieties. A set of 1217 accessions selected from 3000 (3K) re-sequenced rice core collection were comprehensively evaluated for blast resistance in two natural disease nurseries at Lianghe and Bajiao in En-shi area and agronomic traits in disease-free field as well as resistance spectrum of resistant accessions at seedling stage. There were significant differences in response of different accessions to rice blast. Among them, a total of 144 accessions with blast resistance at different growing stages were obtained. Thirty-four accessions with relatively high comprehensive blast resistance were inoculated with 30 diverse isolates, showing that 17 accessions with a broad resistance spectrum (≥70%). According to the investigation of agronomic traits, most resistant accessions had high plant height, low grain yield per plant and poor agronomic traits. Finally, seven accessions with high blast resistance, broad resistant spectrum and relatively good agronomic traits were selected, including IRGA411-1-6-1F-A, YJ 30, Jinzao 47, Quanzhen 10, YN 1353-3, Yunjing 23, and IRAT1047, were identified. Those resistant accessions can be used in mining resistance gene and variety improvement for blast resistance.

关键词

稻瘟病/种质资源/自然诱发/抗谱/优异抗源

Key words

Rice blast/Germplasm/Natural infestation/Resistance spectrum/Excellent resistance resource

引用本文复制引用

李旭升,徐建龙,向小娇,申聪聪,杨隆维,陈凯,王小文,邱先进,朱小源,邢丹英..水稻重测序核心种质资源的稻瘟病抗性鉴定与评价[J].作物学报,2017,43(6):795-810,16.

基金项目

本研究由国家高技术研究发展计划(863 计划)项目(2014AA10A601), 主要粮食作物产业化湖北省协同创新中心开放基金项目(2015MS010, LXT-16-06, LXT-17-02), 深圳市孔雀团队计划(20130415095710361), 湖北省农业科学院合作项目(2015H200014)和湿地生态与农业利用教育部研究中心开放基金项目(KF201403)资助.This study was supported by National High-Tech Research & Development Plan (863 program) (2014AA10A601), Open Fund of Hubei Collaborative Innovation Centre for Grain Industry (2015MS010, LXT-16-06, LXT-17-02), the Shenzhen Peacock Plan (20130415095710361), Collaboration Project of Hubei Academy of Agricultural Sciences (2015H200014), and Open Fund of Research Centre of Ministry of Education for Wetland Ecology & Agronomy Application (KF201403). (863 计划)

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