作物学报2017，Vol.43Issue(6)：811-820,10.DOI:10.3724/SP.J.1006.2017.00811
油菜素内酯合成和信号转导基因在马铃薯块茎贮藏期间的表达变化及对萌芽的影响
Expression Changes of Genes Related to Brassinosteroid Biosynthesis and Sig-nal Transduction during Potato Storage and Its Effect on Tuber Sprouting
摘要
Abstract
To explore the role of brassinosteroid (BR) in tuber sprouting and establish effective sprouting regulation system for seed potato, three varieties and sprouting-inhibitor treatments were used to analyze expression characteristics of nine genes related to BR synthesis, signal transduction and regulation during storage. Effects of BR analogue 24-epibrassinolide (24-eBL) and gib-berellin (GA3) on tuber sprouting were also studied. The transcript levels of four genes involved in BR synthesis increased with prolonging storage time, and the time points at which the expression levels began to increase in variety with short dormancy pe-riod were earlier than those in varieties with middle or long dormancy period. The expression patterns of BRI1 and CYCD3 were similar to those of synthetic genes while BSK and TCH4 remained constant in varieties with middle or long dormancy period. Sprouting-inhibitors stimulated transcripts of those genes to elevate in the earlier stage of storage, then rapidly decline and stay low levels. There was no significant expression change of transcription factor BZR1 in varieties and sprouting-inhibitor treatments during storage. BR analogue 24-eBL was favorable to tuber dormancy release but shoot growth. The tuber weight per plant treated with 24-eBL and GA3 mixture increased by 37.92% to 98.41% compared with CK. The conclusion, BR synthesis and its signal transduction are essential physiological processes from dormancy to sprouting in potato tuber. Mixture of 24-eBL and GA3 can facilitate uniformity of seed potato sprouting, stronger growth and better tuberization.关键词
马铃薯/块茎萌芽/油菜素内酯/基因表达Key words
Potato/Tuber sprouting/Brassinosteroid/Gene expression引用本文复制引用
邹雪,王西瑶,邓孟胜,李立芹,余金龙,丁凡,黄雪丽,彭洁,帅禹,蔡诚诚..油菜素内酯合成和信号转导基因在马铃薯块茎贮藏期间的表达变化及对萌芽的影响[J].作物学报,2017,43(6):811-820,10.基金项目
本研究由现代农业产业技术体系四川薯类创新团队项目(川农业函[2014] 91号), 四川省科技厅公益性育种攻关项目(2016NYZ0032)和绵阳市农业科学研究院创新基金项目(cxjj462016-2019) 资助.This study was supported by the Sichuan Potato Innovation Team Program of Chinese Modern Agricultural Industrial Technology System (Agricultural Department of Sichuan Province Document [2014] No. 91), the Breeding Program for Public Welfare of Science & Technology Department of Sichuan Province (2016NYZ0032), and the Innovation Fund of Mianyang Academy of Agricultural Sciences (cxjj462016-2019). （川农业函[2014] 91号）
