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四倍体马铃薯熟性连锁SCAR标记的开发与验证

李兴翠 李广存 徐建飞 段绍光 卞春松 庞万福 刘杰 金黎平

作物学报2017,Vol.43Issue(6):821-828,8.
作物学报2017,Vol.43Issue(6):821-828,8.DOI:10.3724/SP.J.1006.2017.00821

四倍体马铃薯熟性连锁SCAR标记的开发与验证

Development and Verification of SCAR Marker Linked to Maturity in Tetraploid Potato

李兴翠 1李广存 1徐建飞 1段绍光 1卞春松 1庞万福 1刘杰 1金黎平1

作者信息

  • 1. 中国农业科学院蔬菜花卉研究所/农业部薯类作物生物学和遗传育种重点实验室, 北京 100081
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摘要

Abstract

Maturity is one of the most important quantitative traits in potato. In this study, a maturity linked molecular marker SCAR5-8 was developed using Zhongshu 3, Zhongshu 19 and their F1 segregation population consisting of 221 individuals via reduced-representation sequencing and bulked segregant analysis. A further testing of marker SCAR5-8 was performed using 53 early maturity individuals, 63 late maturity individuals and 70 tetraploid varieties, showing the selection accuracy 87.1% and 81.4% between marker test and phenotyping in segregation population and tetraploid varieties, respectively. Moreover, Pearson's correlation analysis also showed that the marker SCAR5-8 was significantly correlated to maturity trait in potato. The developed SCAR5-8 marker could be used in marker-assisted selection.

关键词

马铃薯/熟性/标记开发/分子标记辅助育种

Key words

Potato/Maturity/Marker development/Marker-assisted selection

引用本文复制引用

李兴翠,李广存,徐建飞,段绍光,卞春松,庞万福,刘杰,金黎平..四倍体马铃薯熟性连锁SCAR标记的开发与验证[J].作物学报,2017,43(6):821-828,8.

基金项目

本研究由国家高技术研究发展计划(863 计划)项目(2013AA102603)和国家现代农业产业技术体系建设专项(CARS-10)资助.This study was supported by the National High Technology Research and Development Program of China (863 Program) (2013AA102603) and the China Agriculture Research System (CARS-10). (863 计划)

作物学报

OA北大核心CSCDCSTPCD

0496-3490

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