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普通小麦近缘种低分子量麦谷蛋白亚基Glu-A3基因的分离和鉴定

董雪 刘梦 赵献林 冯玉梅 杨燕

作物学报2017,Vol.43Issue(6):829-838,10.
作物学报2017,Vol.43Issue(6):829-838,10.DOI:10.3724/SP.J.1006.2017.00829

普通小麦近缘种低分子量麦谷蛋白亚基Glu-A3基因的分离和鉴定

Isolation and Characterization of LMW-GS Glu-A3 in Common Wheat Related Species

董雪 1刘梦 1赵献林 2冯玉梅 1杨燕1

作者信息

  • 1. 内蒙古农业大学生命科学学院植物生物技术功能实验室, 内蒙古呼和浩特 010018
  • 2. 河南省农业科学院小麦研究所/河南省小麦生物学重点实验室/小麦国家工程实验室/农业部黄淮中部小麦生物学与遗传育种重点实验室, 河南郑州 450002
  • 折叠

摘要

Abstract

Low-molecular-weight glutenin subunits (LMW-GS) account for about one-third of wheat seed storage proteins and have great effects on dough extensibility and food processing quality. Common wheat relative species are important genetic re-sources for wheat improvement. In this study, the specific primers of Glu-A3 genes were used to identify the allelic variations of GluA3-1, GluA-2, and GluA3-3 from nine accessions of Triticum dicoccoides, T. dicoccum, T. durum, and T. boeoricum. A total of 15 new haplotypes were identified, including five in GluA3-1, five in GluA3-2, and five in GluA3-3. Among them, two haplotypes each contained nine cysteines that might be good for improvement of wheat quality. The results about Glu-A3 genes in wheat further made clear composition of LMW-GS and provide a basis for selection of parents in wheat quality breeding.

关键词

普通小麦近缘种/低分子量麦谷蛋白亚基/Glu-A3基因/等位变异

Key words

Common wheat related species/LMW-GS/Glu-A3 gene/Allelic variation

引用本文复制引用

董雪,刘梦,赵献林,冯玉梅,杨燕..普通小麦近缘种低分子量麦谷蛋白亚基Glu-A3基因的分离和鉴定[J].作物学报,2017,43(6):829-838,10.

基金项目

本研究由国家自然科学基金项目(31271726), 内蒙古自治区自然科学基金项目(2014MS0338), 内蒙古农业大学优秀青年科学基金项目(2014XYQ-18)和教育部春晖计划项目(Z2009-1-01060)资助.This study was supported by the National Natural Science Foundation of China (31271726), the Natural Science Foundation of Inner Mongo-lia (2014MS0338), the Excellent Young Scientist Foundation of Inner Mongolia Agricultural University (2014XYQ-18), and the Chunhui Project funded by the Ministry of Education (Z2009-1-01060). (31271726)

作物学报

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