作物学报2017，Vol.43Issue(6)：849-854,6.DOI:10.3724/SP.J.1006.2017.00849
一个棉花纤维伸长期优势表达启动子pGhFLA1的克隆与鉴定
Identification of Promoter GhFLA1 Preferentially Expressed during Cotton fiber Elongation
摘要
Abstract
Cotton (Gossypium hirsutum L.) fiber is a perfect model cell for studying cell initiation and growth, so it is critical to find new promoters specifically or preferentially expressed in fiber for functional genomics of fiber and improvement of cotton fiber yield and quality. We found a gene GhFLA1 with very high expression level in fiber elongation stage and cloned its promoter (pGhFLA1). pGhFLA1::GUS was transferred into cotton YZ1 (Gossypium hirsutum) and tobacco (Nicotiana tabacum) to identify its expression pattern in different plant tissues. GUS activity in transgenic plant was obviously detected in fiber from 0 DPA (days post anthesis) to 20 DPA, also in stamens, pistils, sepal, radicle, cotyledon and root, but not in petal, leaf and bract. Quantitative analysis of GUS activity showed that GUS expression level driven by pGhFLA1 was the highest in 20 DPA fiber and 22-fold higher than that in CaMV 35S. In transgenic tobacco, GUS activity was detected in leaf and trichome and reproductive organs like ovary, stigma, anther, bract, petal and seed. In general, the promoter pGhFLA1 fusion with GUS highly expresses in cotton fiber and tobacco trichome, which could be used in fiber functional genomics research and improvement of cotton fiber quality.关键词
棉花(GossypiumhirsutumL.)/GhFLA1/启动子/纤维Key words
Cotton (Gossypium hirsutum L.)/GhFLA1/Promoter/Fiber引用本文复制引用
胡海燕,刘迪秋,李允静,李阳,涂礼莉..一个棉花纤维伸长期优势表达启动子pGhFLA1的克隆与鉴定[J].作物学报,2017,43(6):849-854,6.基金项目
本研究由国家自然科学基金项目(31071465)资助.This study was supported by the National Natural Science Foundation of China (31071465). （31071465）
