主要麻类作物的ITS序列分析与系统进化
Analysis of Internal Transcribed Spacers (ITS) Sequences and Phylogenetics of Main Bast Fiber Crops
摘要
Abstract
Sequences comparison of ribosomal internal transcribed spacer (ITS) could provide evidence for the systematic classi-fication and evolutionary relationships of main bast fiber crops and other species. In this study, the ITS sequences of 32 main bast fiber crops and 11 other species with reference genome sequences were obtained from cloning or GenBank database. The whole gene length, G+C content, and the difference of homologous percentage were analysed using MEGE software. The ITS average lengths of sequences from jute (Corchorus), kenaf (Hibiscus), ramie (Boehmeria nivea) and flax (Linum usitatissimum) were 963, 939, 658, and 686 bp, respectively. And the corresponding G+C contents were 57.87%, 58.03%, 59.05%, and 53.75%, respectively. The variation of jute (Corchorus) concentrated on a region of 220 to 386 bp, kenaf (Hibiscus) on two regions of 206 to 347 bp and 599 to 713 bp, ramie (Boehmeria nivea) on four regions of 158 to 163 bp, 193 to 199 bp, 288 to 333 bp, and 681to 688 bp, and flax (Linum usitatissimum) on five regions of 219 to 229 bp, 235 to 240 bp, 427 to 432 bp, 468 to 484 bp, and 588 to 594 bp. Phylogenetic analysis showed that jute and kenaf shared a relatively close genetic relationship while the others had a far genetic relationship, which is consistent with the relationship of traditional species classification in systematic botany. In study of com-parative genomics, the genome sequecne of cotton might be regarded as a reference for kenaf or jute, and the genome sequence of Populus trichocarpa or Ricinus communis might be regarded as a reference for ramie. We deduced that the evolutionary time of kenaf, jute, ramie and flax could be roughly estimated as 33.7, 65.3, 67.5, and 90.5 million years ago, respectively, showing the longer evolution time the more variation regions of ITS in different species of bast fiber crops in the same genus.关键词
麻类作物/ITS/系统位置/进化关系Key words
Bast fiber crop/ITS/Systematic classification/Evolution relationship引用本文复制引用
张力岚,王俊,万雪贝,徐益,张列梅,方平平,祁建民,张立武..主要麻类作物的ITS序列分析与系统进化[J].作物学报,2017,43(6):862-874,13.基金项目
本研究由高等学校博士学科点专项科研基金新教师类资助项目(20133515120002), 中贝粮油及特色经济作物引育种及新品种示范推广项目(2015I0001), 福建农林大学杰出青年科研基金项目(2012xjj01, xjq201401), 国家现代农业产业技术体系建设专项(CARS-19-E6),农业部东南黄红麻实验观测站项目(农科教发 2011)和福建农林大学大学生创新训练计划项目(201610389149)资助.This study was supported by the Doctoral Program of Higher Education of China (20133515120002), the Introduction Breeding and Varieties Demonstration of Featured Crops between China and Benin (2015I0001), the Distinguished Young Research Fund in Fujian Agriculture and Forestry University (xjq201401), the China Agriculture Research System (nycytx-19-E06), the Experiment Station of Jute and Kenaf in Southeast China (Nongkejiaofa 2011), and the Undergraduate Innovation Training Program in Fujian Agriculture and Forestry University. （20133515120002）
