作物学报2017，Vol.43Issue(6)：940-944,5.DOI:10.3724/SP.J.1006.2017.00940
棉花纤维中木质素的相对分子量
Relative Molecular Weight of Lignin in Cotton Fiber
摘要
Abstract
Molecular weight is one of the important characteristics of polymers, lignin molecular weight and its distribution is one of the contents for studying responses of phenylpropanoid structure, physicochemical characteristics and evaluation of the modi-fied product quality. In this research, the relative molecular weight of lignin extracted respectively by enzyme hydrolysis mild acidolysis and dioxane from mature cotton fibers (Gossypium hirsutum L. acc. TM-1) was studied combined with gel permeation chromatography (GPC). The dioxane lignin (DL) had an average molecular weight (Mw) of 2924 g mol–1, and a number-average molecular weight (Mn) of 2403 g mol–1, which were slightly higher than the enzymatic hydrolysis-mild acidolysis lignin (EMAL, Mw was 2169 g mol–1 and Mn was 1970 g mol–1). The polydispersity coefficient of EMAL was slightly lower than that of DL, showing the higher uniformity in EMAL than in DL. The method of EMAL was more suitable to analyze the relative molecular weight of lignin in cotton fiber. The relative molecular weights of lignin in different cotton varieties, extracted by EMAL, were analyzed, showing that the Mw distribution range of lignin was 938–2169 g mol–1, while Mn was 857–1970 g mol–1 and the polydispersity coefficient was 1.09–1.74 (less than 2). The weight average molecular weight had significantly negative correlation with the micronaire value (P<0.05). The number average molecular weight was negatively correlated with fiber length (P<0.05), and the micronaire value (P<0.01), respectively.关键词
棉花纤维/木质素/相对分子量/酶解-温和酸解/凝胶渗透色谱Key words
Cotton fiber/Lignin/Relative molecular weight/Enzymatic hydrolysis-mild acidolysis/Gel Permeation Chromatog-raphy (GPC)引用本文复制引用
胡文冉,范玲,李晓荣,谢丽霞,杨洋,李波,陈方圆..棉花纤维中木质素的相对分子量[J].作物学报,2017,43(6):940-944,5.基金项目
本研究由新疆维吾尔自治区重点实验室项目(2016D03013)和新疆维吾尔自治区创新人才项目(2014721025)资助.This study was supported by the Key Laboratory Projects of Xinjiang Uygur Autonomous Region (2016D03013) and the Talents Engaging in Scientific and Technological Innovations of Xinjiang (2014721025) （2016D03013）
