植物研究2017，Vol.37Issue(3)：395-401,7.DOI:10.7525/j.issn.1673-5102.2017.03.010
刚毛柽柳ThPP2C基因的克隆和表达分析
Cloning and Expression Analysis of ThPP2C Gene from Tamarix hispida
摘要
Abstract
Protein phosphatase 2C (PP2C) is a kind of serine/threonine protein phosphatase,which is present in the body as monomer.The reversible phosphorylation by PP2C catalytic is an important component of the cell signal transduction,which is involved almost all the physiological and pathological processes.A novel PP2C gene(ThPP2C) was isolated from Tamarix hispida by RT-PCR.The opening reading frame (ORF)length of ThPP2C was 869 bp,encoding 282 amino acid.The relative molecular weight of ThPP2C protein was 30.54 kD,with isoelectric point 7.13.By the real-time RT-PCR,the expressions of ThPP2C were obviously difference in roots and leaves of T.hispida under the treatments(0.4 mol · L-1 NaCl,20％ (w/v) PEG6000,150 μmol · L-1 ABA or 100 μmol · L-1 JA) compared to the control.The expression patterns of ThPP2C were not exactly similarity among these treatments.Particularly,the expression of ThPP2C in leaves of T.hispida after the treatments of NaCl,ABA and JA all were down-regulated.However,it was mainly up-regulated in roots under the treatments of NaCl and JA.With the treatment of ABA,the ThPP2C expression was down-regulated at the early stage and then gradually increased.Different with other three stress,under the treatment of PEG6000,the expressions of ThPP2C were obviously down-regulated in roots.It was not obviously different at the early stress stage in leaves until 48 h,when it was highly induced.Therefore,ThPP2C may play an important role in T.hispida to response the high salt,drought stress and hormones.关键词
蛋白磷酸酶2C(PP2C)/刚毛柽柳/盐胁迫/ABAKey words
PP2C/Tamarix hispida/salt stress/ABA treatment分类
生物科学引用本文复制引用
王培龙,刘中原,张腾倩,唐绯绯,曲冠证,高彩球..刚毛柽柳ThPP2C基因的克隆和表达分析[J].植物研究,2017,37(3):395-401,7.基金项目
黑龙江省教育厅科学技术研究项目(12523017)Science and technology research project of Heilongjiang Provincial Department of Education(12523017) （12523017）
