植物研究2017，Vol.37Issue(3)：453-460,8.DOI:10.7525/j.issn.1673-5102.2017.03.017
水曲柳FmABI5基因非生物胁迫及信号诱导的表达模式分析
Expression Patterns of FmABI5 Gene Induced by Abiotic Stress and Signal in Fraxinus mandshurica
摘要
Abstract
Abscisic acid-insensitive 5 (ABI5) protein was a basic leucine zipper (bZIP) transcription factor containing a basic DNA-binding domain linked to a leucine zipper domain and ABI5 protein response abscisic acid.We studied the FmABI5 expression pattern in signal induction and regulation,and analyzed gene function in metabolic regulation of Fraxinus mandshurica.The ABI5 gene was cloned from F.mandshurica,named FmABI5.The molecular structure of FmABI5 was analyzed by bioinformatics software.Abiotic stress by polyethylene glycol(PEG),salt(NaCl) and low temperature(4℃),and signal induced by abscisic acid(ABA) and gibberellin (GA) were used in the study.Theexpression patterns of FmABI5 induced by abiotic and signal induction were analyzed.By bioinformatics analysis,FmABI5 is 1 455 bp,and contains the complete ORF,encoding 484 amino acids.FmABI5 is a hydrophobic protein without signal peptide but with one transmembrane ability.The alpha helix,extension chain and random coil are distributed throughout the protein.By molecular evolution analysis,the genetic distance of FmABI5 gene between F.mandshurica and Sesamum is relative close.The gene hereditydistance is farther with Solanum tuberosum,S.pennellii,S.torvum and Capsicum annuum,explaining the far genetic relationship with them.After abiotic stress,FmABI5 gene expression levels varied with abiotic stress time with fluctuations,but after 6,48 and 72 h treatment,FmABI5 gene for three abiotic stress treatments were up-regulated,indicating FmABI5 genes are responsive to abiotic stresses.Therefore,the signal induced by exogenous ABA and GA regulate transcription expression of FmABI5 together.关键词
水曲柳/FmABI5/生物信息学分析/非生物胁迫Key words
Fraxinus mandshurica/FmABI5/bioinformatics analysis/abiotic stress分类
农业科技引用本文复制引用
孙爽,赵兴堂,梁楠松,刘颖,于磊,刘春浩,覃俊祺,詹亚光..水曲柳FmABI5基因非生物胁迫及信号诱导的表达模式分析[J].植物研究,2017,37(3):453-460,8.基金项目
国家自然科学基金面上项目(31270697) （31270697）
国家科技支撑项目(2012BAD21B020106) （2012BAD21B020106）
东北林业大学大学生创新创业训练计划项目(201610225233)National Natural Science Foundation of China(31270697) （201610225233）
National Key Technology Support Program(2012BAD21B020106) （2012BAD21B020106）
Innovation and entrepreneurship training program of Northeast Forestry University (201610225233) （201610225233）
