中药材2016，Vol.39Issue(12)：2680-2685,6.DOI:10.13863/j.issn1001-4454.2016.12.002
接种菌根真菌滇重楼幼苗光合作用的光响应曲线
Light Response Curve of Inoculated Paris polyphylla var.yunnanensis Seedling Photosynthesis
摘要
Abstract
Objective:To study Paris polyphylla var.yunnanensis seedling photosynthesis-light response characteristics,and to screen AM fungi that was appropriate to the growth for Paris polyphylla var.yunnanensis,in order to provide a theoretical basis and technical support for the introduction of artificial cultivation and domestication.Methods:The potted inoculation test at room temperature and indoor analysis were used.Take one-year-old Paris polyphylla var.yunnanensis seedling as test materials with consistent growing,and no vaccination for the control group (CK group),to observe physiological characteristics effects of six kinds of arbuscular mycorrhizal (AM) fungi as Gigaspora decipiens,Gigaspora gigantea,Scutellospora dipurpurascens,Racocetra fulgida,Septoglomus deserticola,Acaulospora scrobiculata influenced on Paris polyphylla var.yunnanensis seedling.Results:Paris polyphylla var.yunnanensis can quickly identify each other with AM fungi,forming a good symbiotic relationship.The net photosynthetic rate (Pn),transpiration efficiency (Tr),stomatal conductance(Gs),stomatal limitation value (Ls) of Paris polyphylla var.yunnanensis seedling with vaccinated and unvaccinated AM fungi gradually increased with photosynthetically active radiation (PAR) enhanced,and intercellular CO2 concentration (Ci) were reduced with the increasing of PAR and Pn.Compared with the control group (CK group),after AM fungi induction treatment,there was little effect on Paris polyphylla var.yunnanensis seedling growth and development,but there were some differences of photosynthetic physiology impact among different AM fungi.Overall,after AM fungi invaded Paris polyphylla var.yunnanensis seedling roots and formed symbiont,it can increase the maximum net photosynthetic rate(Pnmax) and light saturation point(LSP),and reduce dark respiration rate (Rd)and light compensation point(LCP) on a degree,thereby enhanced the ability of Paris polyphylla var.yunnanensis seedling to adapt light environment,improved the ability of photosynthesis,and was conducive to the growth.Conclusion:Six kinds of AM fungi on the symbiotic preference for Paris polyphylla var.yunnanensis seedling is different.Considering of Paris polyphylla var.yunnanensis seedling growth,photosynthetic pigment content and photosynthetic characteristics,Scutellospora dipurpurascens and Septoglomus deserticola are the most suitable alternative strains for nurturing Paris polyphylla var.yunnanensis seedling under the experimental condition.关键词
滇重楼/AM真菌/光响应曲线/光响应特征参数/净光合速率/非直角双曲线模型Key words
Paris polyphylla Smith var.yunnanensis (Franch.) Hand.-Mazz./AM fungi/Optical response curve/Light response characteristic parameters/Net photosynthetic rate/Non-rectangular hyperbolic model分类
医药卫生引用本文复制引用
潘兴娇,郭冬琴,张杰,冯源,韦正鑫,周浓..接种菌根真菌滇重楼幼苗光合作用的光响应曲线[J].中药材,2016,39(12):2680-2685,6.基金项目
国家自然科学基金(81260622) （81260622）
云南省教育厅科学研究基金重点项目(2012Z119) （2012Z119）
