北华大学学报（自然科学版）2017，Vol.18Issue(2)：182-185,4.DOI:10.11713/j.issn.1009-4822.2017.02.008
野山参与园参rDNA-ITS序列比较分析
Comparative Analysis of Wild Ginseng and Cultivated Ginseng in rDNA-ITS Sequence
摘要
Abstract
Objective The difference of rDNA-ITS sequences between wild ginseng and cultivated ginseng was compared for building DNA molecular identification method.MethodThe total DNA of wild ginseng and cultivated ginseng was extracted.ITS sequences of both were amplified and analyzed by ClustalX software.ResultsThe PCR results of wild ginseng and cultivated ginseng obtained two bands,700 bp and 500 bp respectively.There was no difference between wild ginseng and cultivated ginseng in the sequencing of 700 bp band and only 38% similarity in the sequencing of 500 bp band.ConclusionThis results provided the experimental bases for the identification.关键词
野山参/园参/rDNA-ITS序列/鉴别/质量评价Key words
wild ginseng/cultivated ginseng/rDNA-ITS sequence/identify/quality assessment分类
医药卫生引用本文复制引用
王晶,付宝玉,宋卓,尹旭东,孙立伟,麻锐..野山参与园参rDNA-ITS序列比较分析[J].北华大学学报（自然科学版）,2017,18(2):182-185,4.基金项目
国家自然科学基金项目(81373932) （81373932）
吉林省大学生创新创业训练计划项目(201510201058) （201510201058）
吉林省教育厅科学技术研究项目(201676) （201676）
吉林省科技发展计划项目(20150520138JH). （20150520138JH）
