北华大学学报（自然科学版）2017，Vol.18Issue(2)：205-208,4.DOI:10.11713/j.issn.1009-4822.2017.02.014
HO-1在慢性鼻-鼻窦伴鼻息肉患者鼻息肉组织中的表达及调节机制研究
Expression and Regulation Mechanism of HO-1 in Nasal Polyp Tissues of Patients with Chronic Rhinosinusitis with Polyps
摘要
Abstract
ObjectiveTo investigate the expression and regulation of heme synthase 1 (HO-1) in nasal polyp tissues of patients with chronic rhinosinusitis with polyps (CRSwNP).MethodNasal polyp tissues of 50 cases of chronic rhinosinusitis with polyps were taken for the investigation and the uncinate process mucosa tissues of 30 cases with simple nasal septum deviation were collected as the control group at the same time.Real-time fluorescence quantitative polymerase chain reaction (RT-PCR) and immunohistochemistry and Western blot were used to detect the expression of HO-1 in the nasal polyp and the uncinate process mucosa tissues.Moreover,the uncinate process mucosa tissues were taken and cultured in vitro for the detection of different cytokines (IL-17A and TGF-β1) and HO-1 mRNA in the nasal mucosa stimulated by dexamethasone (DEX).ResultsThe expression level of mRNA HO-1 in nasal polyps was significantly higher than that in the mucosal tissues (P<0.05).HO-1 positive cells in nasal polyps were mostly distributed in the submucosal glands,epithelial cells and inflammatory cells;HO-1 positive cells were significantly more than those in the control group (P<0.05).The expression level of HO-1 protein in nasal polyps was significantly higher than that in control group (P<0.05).IL-17A could significantly enhance the level of mRNA HO-1 expression,TGF-beta 1 and DEX could significantly reduce the level of mRNA HO-1 expression,and the stimulation of IL-17A,TGF-beta 1 and DEX was concentration-dependent.The expression levels of HO-1 mRNA in the combined DEX and IL-17A-stimulated group and the combined DEX and TGF-β1-stimulated group were significantly lower those in the IL-17A-stimulated group (P<0.05).ConclusionHO-1 as an antagonistic antioxidant,can be up-regulated in a feedback way in the process of oxidative stress of the nasal polyp tissues,and glucocorticoids can inhibit its up-regulated expression.关键词
鼻窦炎/鼻息肉/HO-1/氧化应激Key words
Sinusitis/nasal polyps/HO-1/oxidative stress分类
医药卫生引用本文复制引用
杨军,田进强..HO-1在慢性鼻-鼻窦伴鼻息肉患者鼻息肉组织中的表达及调节机制研究[J].北华大学学报（自然科学版）,2017,18(2):205-208,4.基金项目
江苏省卫生厅科技计划项目(H2014-30). （H2014-30）
