北京大学学报（自然科学版）2017，Vol.53Issue(2)：387-396,10.DOI:10.13209/j.0479-8023.2016.122
铅锌矿区土壤真菌响应重金属污染的群落组成变化
Characterization of Soil Fungal Community in Response to Heavy Metal Pollution in Lead-Zinc Mining Area
摘要
Abstract
Heavy metal contamination is one of the most concerned global environmental problems and the soil heavy metal contamination is especially severe in lead-zinc mining areas in China.In order to study the soil fungal community composition changes responding to different degree of heavy metal pollution,soil samples from the lead-zinc mine field of Yunnan Mengnuo were studied.5 samples from the heavy metal pollution soil (HP) and other 4 from the low pollution (LP),based on the cluster analysis of heavy metal contents and the physical and chemical properties of the sample were analyzed.Genomic DNA of the soil samples were extracted and the Internal Transcribed Spacer (ITS) genes were sequenced by the high-through sequencing Illumina MiSeq.The fungal communities at different taxonomic levels (Phylum,Class,Order,Family,Genus and Species) were compared.In HP samples,the abundance of unclassified fungi were the highest,then followed by Aspergillus,Un--s-Clavulinaceae sp.and Un--s-fungal sp.ARIZ L453 respectively.In LP samples,the unclassify fungi were also high,but less than HP.The relative abundance of fungi from high to low was Geastrum,Aspergillus and Mortierella.The Representational Difference Analysis (RDA) showed that different heavy metals influence fungal community diversity and the concentrations of Pb was significantly correlated with fungal community.关键词
铅锌矿/重金属污染/高通量测序/真菌群落组成Key words
lead-zinc mining/heavy metal pollution/high-throughput sequencing/fungal community分类
环境科学引用本文复制引用
杨金水,杨扬,孙良明,刘伟杰,曾远,邓春萍,邢冠岚,袁红莉..铅锌矿区土壤真菌响应重金属污染的群落组成变化[J].北京大学学报（自然科学版）,2017,53(2):387-396,10.基金项目
中国地质调查局地质调查工作项目(12120113015200)资助 （12120113015200）
