吉林大学学报（医学版）2017，Vol.43Issue(2)：230-235,封2,前插1,8.DOI:10.13481/j.1671-587x.20170204
小鼠体内气管滴注纳米二氧化硅对体内主要脏器的影响
Effects of SiO2 nanoparticles by intratracheal instilation on major organs of mice in vivo
摘要
Abstract
Objective:To study the effects of SiO2 nanoparticles on the organs of mice in vivo after intratracheal instillation, and to provide the basis for safety evaluation of SiO2 nanoparticles. Methods:Forty female BALB/c mice aged 6-8 weeks were randomly divided into control group (saline), low dose of SiO2 group (7 mg·kg-1), middle dose of SiO2 group (21 mg·kg-1), and high dose of SiO2 group (35 mg·kg-1).1 and 15 d after five times of non-exposed intratracheal instilation infection (once every 3 d), the mice were sacrificed and the left lungs,the right kidneys, livers, hearts and spleens were collected and embedded in paraffin.The morphology of tissue sections was observed under light microscope after hematoxylin-eosin (HE) staining.The eyeball blood was obtained and the biochemical indicators of liver and kidndy functions were detected.Results:Compared with control group, there were alveolar interval thickening, inflammatory cell infiltration, and a small amount of small arterial thrombosis in the lungs;granulomatous inflammatory cell infiltration and a small amount of focal necrosis of liver cells in the livers;red pulp enlargement, hyperemia, and more visibly scattered megakaryocytes in the spleens in SiO2 nanoparticles groups in a dose-dependent manner, especially in middle and high doses of SiO2 groups.After 15 d of injection, the damages alleviated with the prolongation of time.There was some inflammatory cell infiltration in the kidney tissue of the mice in SiO2 nanoparticle groups.The biochemical indicator detection results showed that alanine aminotransferase(ALT) and aspartate transaminase(AST) levels in SiO2 nanoparticles groups varied, suggesting the liver cell damages were at different degrees;the changes of urea nitrogen(BUN) and creatinine(Cr) levels in SiO2 nanoparticle groups remindered the kidney function alteration, but there were no obvious dose-and time-dependent effects.Conclusion:Intratracheal instillation of SiO2 nanoparticles can influence the major organs of the mice and mainly displays in the inflammation and injuries in the lung, liver, and spleen.关键词
纳米二氧化硅/气管滴注/组织病理学/炎症Key words
SiO2 nanoparticles/intratracheal instillation/histopathology/inflammation分类
医药卫生引用本文复制引用
杨曼,孙小铃,王继,梁宝璐,李艳博,荆黎,孙志伟..小鼠体内气管滴注纳米二氧化硅对体内主要脏器的影响[J].吉林大学学报（医学版）,2017,43(2):230-235,封2,前插1,8.基金项目
国家自然科学基金资助课题(81402709) （81402709）
