吉林大学学报（医学版）2017，Vol.43Issue(2)：241-244,前插1,5.DOI:10.13481/j.1671-587x.20170206
出生后小鼠牙不同发育期DKK1表达的形态学特点及其意义
Morphological characteristics of expressions of DKK1 in different periods of tooth development in postnatal mice and their significances
摘要
Abstract
Objective:To study the expression characteristics of Dickkopf1 (DKK1) in different time and space during tooth development of the postnatal mice, and to provide the theoretical basis for clarifying the mechanism of Wnt signaling pathway in regulating the tooth development.Methods:The postnatal Kunming mice at days 0.5, 6.5, 12.5, 18.5, 24.5, and 30.5 respectively after birth were selected and divided into various groups by time,three in each group.The mice in each group were sacrificed and the paraffin sections of mandibular bone including the first molar were prepared at the thickness of 5 μm, followed by HE staining and immunohistochemical staining in order to detect the expressions of DKK1 in tooth tissue and periodontal tissue.Results:At 0.5 d after birth, the mandibular first molar tooth germ was in the bell stage.At 6.5 d the enamel development of mandibular first molar was almost completed, and the epithelium root sheath extended to the root direction.At 12.5 d the dentin development of crown was completed, with the root formatted about 1/3. At 18.5 d the root had formatted about 2/3.At 24.5 d the root had reached the full length.At 30.5 d the apical foramen was narrow, and the root development was basically completed.There was no DKK1 expression at 0.5 d, but it expressed in the odontoblasts and predentin at 6.5 d. From days 12.5 to 30.5,the expressions of DKK1 were positive in periodontal ligament, alveolar bone, and cellular cementum as odontoblasts, which were gradually increased with the prolongation of time.However, no expression of DKK1 was detected in the pulp.Conclusion:DKK1 shows regular expressions at different tooth developmental stages after birth, suggesting its potential role in the growth of dentin and periodontal tissues.关键词
DKK1/牙发育/牙周组织/免疫组化染色/苏木素-伊红染色Key words
DKK1/dental development/periodontal tissue/immunohistochemistry/hematoxylin and eosin staining分类
医药卫生引用本文复制引用
郭小凯,包幸福,杨军星,王玉琢,李雨桐,胡敏..出生后小鼠牙不同发育期DKK1表达的形态学特点及其意义[J].吉林大学学报（医学版）,2017,43(2):241-244,前插1,5.基金项目
国家自然科学基金资助课题(81470764) （81470764）
吉林省卫计委青年科研基金资助课题(2015Q003) （2015Q003）
吉林大学白求恩计划项目资助课题(2015321) （2015321）
吉林省科技厅科技发展计划项目资助课题(20160520156JH) （20160520156JH）
