磁共振成像2017，Vol.8Issue(1)：45-50,6.DOI:10.12015/issn.1674-8034.2017.01.011
新生猪缺氧缺血脑损伤后基底节乳酸代谢及其转运体表达的研究
Changes in lactate and associated transporters expression in basal ganglia following hypoxic-ischemic brain injury in piglets
摘要
Abstract
Objective:To investigate the expression characteristics of lactate and associated transporters in basal ganglia following hypoxic-ischemic reperfusion brain injury in a piglet model.Materials and Methods:A total of 35 healthy piglets (3-5 days old,1.0-1.5 kg) were selected.They were divided into control (n=5) and hypoxic-ischemic (HI) model groups (n=30).The HI model group was further divided into six groups according to 1H-magnetic resonance spectroscopy (1H-MRS)scan times after HI (0-2 h,2-h,6-12 h,12-24 h,24-48 h and 48-72 h;n=5/group).The HI model was established by bilateral common carotid artery occlusion and simultaneous hypoxia treatment for 40 min.Piglets in the control group received the same surgical procedure without the hypoxia-ischemia process.1H-MRS imaging was performed at various time points after HI.The right basal ganglia was the region of interest (ROI) in 1H-MRS imaging for which data was processed by LcModel software.Animals were euthanized immediately after the last scan and the whole brain was quickly removed and bilateral hemispheres separated.The right hemisphere was used for the pathological examination and immunohistochemical staining of monocarboxylate transporters (MCTs).ANOVA analyses were conducted.P＜0.05 represented statistical significance.Results:(1) The lactate level became reduced after an initial increase,with the maximal level occurring around 2-6 h following HI.(2) The expression of both MCT-2 and MCT-4 in the basal ganglia initially reached a peak value at 12-24 h and decreased thereafter and they were significantly different at 12-24 h after HI compared to the control group and the other time points of the HI model group (P＜0.05).Conclusions:These results indicate that lactate content has potential to regulate the expression of its related transporters in neuronal and glial cell and they have a synergistic effect on the energy metabolism following hypoxic-ischemic reperfusion brain injury.关键词
脑/缺氧缺血/乳酸/新生猪/磁共振波谱学Key words
Brain/Hypoxia-ischemia/Lactate/Piglet/Magnetic resonance spectroscopy分类
医药卫生引用本文复制引用
郑阳,王晓明..新生猪缺氧缺血脑损伤后基底节乳酸代谢及其转运体表达的研究[J].磁共振成像,2017,8(1):45-50,6.基金项目
国家自然科学基金(编号:30570541、30770632、81271631) This study was supported by National Natural Science Foundation of China (NO.30570541,30770632,81271631). （编号:30570541、30770632、81271631）
