重庆医学2017，Vol.46Issue(9)：1156-1158,3.DOI:10.3969/j.issn.1671-8348.2017.09.002
吸烟诱导肺气肿大鼠血清AECA的表达及甲基波尼松龙的干预影响
Expression of serum anti-endothelial cell antibody in emphysema rats induced by smoking and intervention effect of methylprednisolone
摘要
Abstract
Objective To investigate the expression of anti-endothelial cell antibody AECA in the emphysema rats induced by smoking and to analyze the intervention effect of methylprednisolone on it.Methods Thirty-nine rats were randomly divided into the control group,smoking rat emphysema model group (model group) and methylprednisolone intervention group (intervention group).The model group and intervention group conducted the 1-month passive smoking by smog exposure.The intervention group was intraperitoneally injected by methylprednisolone(once daily,6 d per week).After exposing to smog for 90 d,the differences of serum AECA level,IL-8,MMP-9 and TNF-α level in bronchoalveolar lavage fluid(BALF),lung MLI and mean alveolar number (MAN) were compared among groups.Results Compared with the control group and theintervention group,the level of serum AECA in the model group was significantly increased (P＜0.05);Compared with the control group and intervention group,the IL-8,TNF-α and MMP-9 levels of BALF in the model group were also increased(P＜0.05).Conclusion ACEA participates in the smoking induced emphysema formation;methylprednisolone may decrease the level of AECA and cell inflammatory factors and affects the emphysema formation.关键词
肺气肿/甲基波尼松龙/抗血管内皮细胞抗体/支气管肺泡灌洗液/肿瘤坏死因子α/白细胞介素8/基质金属蛋白酶9Key words
pulmonary emphysema/methylprednisolone/anti-endothelial cell antibodies/bronchoalveolar lavage fluid/tumor necrosis factor-alpha/interleukin-8/matrix metalloproteinase 9分类
医药卫生引用本文复制引用
张璐,王中新,马丽,张程,张湘燕..吸烟诱导肺气肿大鼠血清AECA的表达及甲基波尼松龙的干预影响[J].重庆医学,2017,46(9):1156-1158,3.基金项目
国家自然科学基金资助项目(81060006) （81060006）
十二五国家科技支撑计划课题资助项目(2013bai09b09) （2013bai09b09）
贵州省科学技术基金资助项目[黔科合j字(2011)2251]. （2011）
