重庆医学2017，Vol.46Issue(9)：1159-1162,1167,5.DOI:10.3969/j.issn.1671-8348.2017.09.003
氢气对大鼠局灶性脑缺血再灌注损伤内质网应激及神经细胞凋亡的影响
Effect of hydrogen gas on endoplasmic reticulum stress and neural cell apoptosis in rats with cerebral ischemia-reperfusion injury
摘要
Abstract
Objective To investigate the effects of inhaling high concentration of hydrogen gas on the expressions of endoplasmic reticulum stress(ERS) related protein glucose regulated protein 78 (GRP78),Caspase-12 and the neural cell apoptosis and related proteins Bcl-2 and Bax in the rats with focal cerebral ischemia reperfusion(I/R) injury.Methods Seventy-two healthy SPF male Sprague-Dawley rats were selected and then randomly divided into the control group(Ⅰ:without any treatment),sham operation group (Ⅱ),cerebral IRI group (Ⅲ) and hydrogen gas treatment group (Ⅳ),18 cases in each group.Focal cerebral ischemia reperfusion injury (IRI) model was induced by using the suture-occluded method.The neurological deficits score (NDS) was assessed at 24 h after cerebral reperfusion in four groups.The cerebral infarction severity and size were detected by TTC staining and neuronal apoptosis of brain cortex were tested by TUNEL technique.The apoptosis index (AI) was calculated.Then the expressions of GRP78,Caspase-12,Bcl-2 and Bax were assessed by Western blot and immunohistochemistry.Results As compared with the group Ⅰ and Ⅱ,NDS score,cerebral infarction size,AI and the expressions of GRP78,Caspase-12 and Bax in cerebral cortex in the group Ⅲl and Ⅳ were significantly increased,while the expression of Bcl-2 in cerebral cortex was markedly decreased(P＜0.05);compared with the group Ⅲ,NDS score,brain infarction size,AI and the expression of Caspase-12 and Bax in cerebral cortex in the group Ⅳ were markedly decreased,while the expressions of GRP78 and Bcl-2 were dramatically increased (P＜0.05).Conclusion Inhaling high concentration of hydrogen gas has a certain protective effect on cerebral IRI in rats through increasing endoplasmic reticulum GRP78 protein expression after IRI and inhibiting Caspase-12 activation,thus inhibiting ERS and promoting the repair function of endoplasmic reticulum.关键词
氢/脑缺血/再灌注损伤/细胞凋亡/内质网/应激Key words
hydrogen/brain ischemia/reperfusion injury/apoptosis/endoplasmic reticulum/stress分类
医药卫生引用本文复制引用
夏裕宁,李雪梅,袁楠楠,张鑫磊,谭永星..氢气对大鼠局灶性脑缺血再灌注损伤内质网应激及神经细胞凋亡的影响[J].重庆医学,2017,46(9):1159-1162,1167,5.基金项目
国家自然科学基金资助项目(81260206) （81260206）
广西壮族自治区自然科学基金资助项目(2013GXNSFAA019155,2015GXNSFAA139130) （2013GXNSFAA019155,2015GXNSFAA139130）
广西壮族自治区卫生厅课题(Z2009038,Z2013497,Z2012402) （Z2009038,Z2013497,Z2012402）
广西医学科学实验中心开放课题(KFJJ2011-07). （KFJJ2011-07）
