大豆科学2017，Vol.36Issue(2)：187-192,6.DOI:10.11861/j.issn.1000-9841.2017.02.0187
大豆对大豆花叶病毒SC18株系的抗性遗传和基因定位
Genetic Analysis and Mapping of Soybean Mosaic Virus Resistance Genes to SC18 in Soybean
摘要
Abstract
Soybean mosaic virus (SMV) disease is a major soybean disease in soybean production in China,and SMV strain SC18 is one of the most popular strain in soybean producing regions of southern and northeastern China,and it is also coming up in Huanghuai valleys.In this study,progenies derived from the resistance (R)soybean cultivar × susceptible (S) soybean cultivar were inoculated with SC18,all F1 plants were resistant to SC18,the segregation of R and S individuals fitted a 3∶1 ratio in the F2 population,and fitted a 1∶2∶1 ratio in the segregation of resistant,segregating and susceptible in the F2∶3 lines,the results suggested that all the 5 resistance parents Zhongzuo 00-683,Bindou 95-20,Dongda 2,Zhongpin 661 and RN-9 possess one dominant resistance gene to SC18.The segregation pattern of F2 individuals from the resistance (R) soybean cultivar × resistance (R) soybean cultivar ‘ Zhongzuo 00-683 × Dongda 2'fitted a 15R∶ 1S ratio,support the hypothesis that two possible independent dominant genes confer resistance to SC18 in Zhongzuo 00-683 and Dongda 2.And all F1,F2,and F2∶3 progenies derived from R × R cross ‘ Zhongzuo 00-683 × Bindou 95-20’ were resistant to SC18,showed that SC18 resistance genes in Zhongzuo 00-683 and Bindou 95-20 are allelic.Further,the SC18 resistance gene in RN-9 (designated Rsc18) was mapped on soybean chromosome 6 (linkage group C2) between the two SSR markers Satt286 and Satt277 Using RN-9 × 7605 recombinant inbred lines (RILs),with the genetic distance 6.12 and 4.69 cM,respectively.关键词
大豆/大豆花叶病毒(SMV)/遗传分析/基因定位Key words
Soybean/Soybean mosaic virus/Genetic analysis/Gene mapping分类
农业科技引用本文复制引用
李凯,任锐,王涛,高乐,落金艳,刘士超,智海剑,盖钧镒..大豆对大豆花叶病毒SC18株系的抗性遗传和基因定位[J].大豆科学,2017,36(2):187-192,6.基金项目
国家自然科学基金(31671718,31571690) （31671718,31571690）
中央高校基本科研业务费(Y0201600115) （Y0201600115）
现代农业产业技术体系(CARS-04) （CARS-04）
转基因生物新品种培育科技重大专项(2016ZX08004-004) （2016ZX08004-004）
江苏省现代作物生产协同创新中心(JCIC-MCP). （JCIC-MCP）
