大豆科学2017，Vol.36Issue(2)：193-198,6.DOI:10.11861/j.issn.1000-9841.2017.02.0193
利用SRAP分子标记鉴定内蒙古栽培大豆与野生大豆杂交后代真实性
The Authentic Evaluation on Hybrids of Wild Soybean and Cultivated Soybean Based on SRAP Marker in Inner Mongolia
摘要
Abstract
In this study,the hybrids of wild soybean and cultivated soybean were analyzed by SRAP marker to select out the true hybrids and build fingerprint.15 primer combinations of SRAP with characteristics of rich polymorphism,clear bands and good repeatability were selected from 234 primer combinations at first,then primers were selected to bands specific for the male parent but absent from the female,10 primers were selected and applied to identify the hybrids.There were 48 progenies with those specific bands,which were therefore identified as true hybrids.The 15 primer combinations were used in the PCR amplification,347 DNA bands were obtained,265 of which bands were polymorphic,amounted to 76.37％ of the total amplified bands.The result showed that SRAP markers could be used as effective molecular markers for the authenticity identification of the progenies of soybean,the selected 15 primer combinations could be effectively applied to SRAP analusis of the germplasm resources of hybrids.关键词
SRAP标记/杂交大豆/引物筛选/杂种鉴定Key words
SRAP marker/Hybrids/Primer screening/Hybrids identification分类
农业科技引用本文复制引用
刘雪骄,王明玖,索荣臻..利用SRAP分子标记鉴定内蒙古栽培大豆与野生大豆杂交后代真实性[J].大豆科学,2017,36(2):193-198,6.基金项目
国家重点研究发展计划(2016YFC0500605). （2016YFC0500605）
