大豆科学2017，Vol.36Issue(2)：199-207,9.DOI:10.11861/j.issn.1000-9841.2017.02.0199
基因拷贝数变异分析法研究大豆胞囊线虫病抗性位点qSCN3-3抗性相关基因
Research on the Resistance Genes Related to Soybean Cyst Nematode Resistance Loci qSCN3-3 with the Copy Number Variation Analysis
摘要
Abstract
Gene copy number variants (CNVs) can cause the difference in soybean resistance to soybean cyst nematode (SCN).In this study,four soybean germplasms including Dongnong L-10(resistant to SCN),Heinong 37,Suinong 10 and Suinong 14 (susceptible to SCN) were selected to analyze the CNVs of 27 genes in the genomic region of SCN resistance related quantitative trait loci(QTL) ‘ qscn3-3’ using the method of real-time quantitative PCR.The result of F test showed that the copy number of 15 genes were different among four soybean germplasms.A total of 9 genes showed significant difference in copy number between Dongnong L-10 and the other three susceptible germplasms.It was speculated that its function was accomplished by coding disease-resistant protein and leucine rich protein for SCN resistance.关键词
大豆/胞囊线虫抗性/荧光定量PCR/拷贝数变异Key words
Soybean/Cyst nematode resistance/Copy number variation/Real-time quantitative PCR分类
农业科技引用本文复制引用
朱治佳,赵雪,罗健,臧紫薇,韩英鹏,李文滨..基因拷贝数变异分析法研究大豆胞囊线虫病抗性位点qSCN3-3抗性相关基因[J].大豆科学,2017,36(2):199-207,9.基金项目
省自然科学基金面上项目(C2015011) （C2015011）
国家自然科学基金(31301339,31671717) （31301339,31671717）
黑龙江东北农业大学学术骨干计划项目(15XG04) （15XG04）
东北农业大学青年才俊计划项目(14QC27). （14QC27）
