大豆科学2017，Vol.36Issue(2)：305-308,4.DOI:10.11861/j.issn.1000-9841.2017.02.0305
EDTA对大豆脂氧酶的活性和催化体系的影响
Effects of EDTA on the Soybean Lipoxygenase Catalytic System
摘要
Abstract
The effects of EDTA on the stability and catalytic behavior of lipoxygenase(LOX) were studied in this paper.The results showed that EDTA could improve the stability of LOX significantly,and it could retain high activity(80％ or more) in a long time(30 days) at room temperature.EDTA can greatly improve the catalytic efficiency of LOX catalyzed on linoleic acid,so that the reaction process can be reduced to 1/3 of the original.But it would inhibit the catalytic activity when the substrate is soybean oil.Spectral experiments show that EDTA induces only a fine tuning of the local conformation of LOX,and does not involve the change of the secondary structure or tertiary structure.EDTA has great application value in preserving liquid LOX and catalytic oxidation of linoleic acid.关键词
脂氧酶/EDTA/稳定性/催化Key words
Lipoxygenase/EDTA/Stability/Catalysis分类
生物科学引用本文复制引用
沈洁,田丹,蔡燕,陈娇,赵勤,吴锦明..EDTA对大豆脂氧酶的活性和催化体系的影响[J].大豆科学,2017,36(2):305-308,4.基金项目
江苏省青年自然科学基金(BK20150401,BK20150402) （BK20150401,BK20150402）
江苏省高校自然科学研究面上项目资助(15KJB530010,15KJB150024). （15KJB530010,15KJB150024）
