电化学2017，Vol.23Issue(1)：53-58,6.DOI:10.13208/j.electrochem.160829
基于WS2量子点材料固定葡萄糖氧化酶的直接电化学及其生物传感研究
Direct Electrochemistry of Glucose Oxidase Based on WS2 Quantum Dots and Its Biosensing Application
摘要
Abstract
In this study,a novel electrochemical glucose biosensor has been developed by immobilizing glucose oxidase (GOx) on t ungsten disulfide quantum dots (WS2 QDs) on the surface of glassy carbon electrode (GCE).Transmission electron microscopy,UV-vis spectroscopy and cyclic voltammetry were employed to characterize the morphology,structure,and electrochemical behaviors of the as-prepared WS2 QDs and the biofilm modified electrode.The results suggested that the WS2 QDs could accelerate the electron transfer between the electrode and the immobilized enzyme,which enabled the direct electrochemistry of GOx without any electron mediator.Besides,the immobilized GOx in WS2 QDs film exhibited excellent electrocatalytic activity toward oxidation of glucose due to the excellent biocompatibility of the WS2 QDs.The proposed GOx/WS2 QDs biofilm electrode exhibited a linear response to glucose concentration in the ranges of 25 ～ 100 μmol· L-1 and 100 ～ 600 μmol· L-1,and the detection limit of the biosensor was as low as 5.0 μmol· L-1.The biosensor also exhibited good selectivity,reproducibility and long-term stability,which might be potenially used for the detection of the real samples.关键词
WS2量子点/葡萄糖氧化酶/直接电化学/生物传感器Key words
tungsten disulfide quantum dots/glucose oxidase/direct electrochemistry/biosensor分类
化学化工引用本文复制引用
李晨露,彭花萍,黄种南,盛依伦,吴佩文,林新华..基于WS2量子点材料固定葡萄糖氧化酶的直接电化学及其生物传感研究[J].电化学,2017,23(1):53-58,6.基金项目
国家自然科学基金项目(No.21405015,No.81400805)、福建省自然科学基金资助项目(No.2014J05092,No.2016J01449)及大学生创新创业训练项目基金(No.C1634)资助 （No.21405015,No.81400805）
