福建农业学报2017，Vol.32Issue(1)：1-6,6.DOI:10.19303/j.issn.1008-0384.2017.01.001
嗜水气单胞菌脂肪酸生物合成途径相关蛋白的耐药性
Antibiotic-resistance Related to Proteins Associated with Fatty Acid Biosynthesis in Aeromonas hydrophila
摘要
Abstract
Widely exists in water and soil,Aeromonas hydrophila is a commonpathogen for human,livestock and fish.It was previously observed in our lab that the expression of the proteins associated with the fatty acid biosynthesis in A.hydrophilarose under the stress of chlortetracycline.But the specific properties and functions were not understood.Therefore,this study was designed to clone and express those potentially important proteins,including acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (AccA),acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (AccD),3-oxoacyl-[acyl-carrier-protein] synthase I (FabB),and malonyl coAacyl carrier protein transacylase (FabD) for further investigation.By comparing the survival rates of high-expression strains of A.hydrophila under chlortetracycline stress,the drug-resistance relating to the proteinsin the fatty acid biosynthesispathway was analyzed.The results showed that the high-expression proteins,AccD,FabB and FabD,could indeed significantly increase the survival rate of the pathogen under chlortetracycline stress,while AccA did so only during the initial stage.Consequently,a critical role of these proteins involving the bacterial drug-resistance was clearly demonstrated.With the finding,further study to unveil the mechanism of antibiotic-resistance of the pathogen is in order.关键词
嗜水气单胞菌/脂肪酸生物合成/耐药性Key words
Aeromonas hydrophila/fatty acid biosynthesis/resistance分类
生物科学引用本文复制引用
胡文杰,张香玉,吴倩,林梅贵,黄小芳,林向民..嗜水气单胞菌脂肪酸生物合成途径相关蛋白的耐药性[J].福建农业学报,2017,32(1):1-6,6.基金项目
国家自然科学基金项目(31200105、31470238) （31200105、31470238）
