福建农业学报2017，Vol.32Issue(1)：7-11,5.DOI:10.19303/j.issn.1008-0384.2017.01.002
猪巨细胞病毒福建株gB基因的克隆和序列分析
Cloning and Sequencing of gB Gene from Porcine Cytomegalovirus Isolated in Fujian
摘要
Abstract
Based upon the characterization of gB gene retrieved from GenBank,specific primers were selected to amplify the gB gene of porcine cytomegalovirus (PCMV) FJ01 isolated in Fujian.The target PCR fragments were then cloned and sequenced.The gB gene was found to have 2 580 nucleotides,encoding 859 amino acids.Its homologies on nucleotide and amino acid sequences with the data at GenBank were greater than 98.3 ％ and 97.9 ％,respectively.In addition,nearly half of the PCMV isolates had a same ACA nucleotide deletion in their gB genes that correlated to the phylogenetic evolution of the isolates,except ZZ strain.关键词
猪巨细胞病毒/gB基因/克隆/序列分析Key words
porcine cytomegalovirus/gB gene/clone/sequence analysis分类
农业科技引用本文复制引用
陈如敬,魏宏,周伦江,吴学敏,车勇良,王晨燕,王隆柏,黄晓凤,严山,刘玉涛..猪巨细胞病毒福建株gB基因的克隆和序列分析[J].福建农业学报,2017,32(1):7-11,5.基金项目
福建省畜禽疫病防控技术重大研发平台项目(2014N2003) （2014N2003）
福建省科技计划项目——省属公益类科研院所基本科研专项(2014R1023-16、2014R1023-13) （2014R1023-16、2014R1023-13）
