福建农业学报2017，Vol.32Issue(1)：12-16,5.DOI:10.19303/j.issn.1008-0384.2017.01.003
福建省羊支原体性肺炎分子流行病学研究
A Molecular Epidemiological Study on Mycoplasma Pneumonia of Sheep and Goats in Fujian
摘要
Abstract
The molecular epidemiology on the mycoplasma pneumonia of sheep and the contagious caprine pleuropneumonia of goats in Fujian were investigated.A total of 61 samples from the goats with suspected infection of the diseases were collected from 6 regions in the province during 2014-2016.PCR with the mycoplasmatales primer as well as the specific primers for Mycoplasma ovipneumoniae,Mycoplasma mnycoides subsp.capri,Mycoplasma capricolum Subsp.Capripneumonia,Mycoplasma capricolum subsp.capri,Mycoplasma arginin,Acholeplasma laidlawii and Mycoplasma agalactiae were applied for the detection.Part of the PCR positive clinical samples were selected to isolate Mycoplasma for subsequent cloning and sequencing based on their 16S rRNA.The detection rates on M.ovipneumoniae,M.mycoides subsp.capri,A.laidlaw,ii and M.arginine were 73.77％ (45/61),6.56％ (4/61),6.56％ (4/61) and 3.28％ (2/61),respectively.The results suggested that the suspected contagious caprine pleuropneumonia in the province were mycoplasma pneumonia of sheep and goats caused mainly by M.ovipneumoniae.The finding would lead to effective prevention and treatment measures for the diseases.关键词
羊支原体性肺炎/分子流行病学/福建省Key words
mycoplasma pneumonia of sheep and goats/molecular epidemiology/Fujian Province分类
农业科技引用本文复制引用
江锦秀,林裕胜,游伟,江斌,胡奇林..福建省羊支原体性肺炎分子流行病学研究[J].福建农业学报,2017,32(1):12-16,5.基金项目
福建省科技计划项目——省属公益类科研院所基本科研专项(2014R1023-6) （2014R1023-6）
福建省科技创新平台建设项目(2014N2003-5) （2014N2003-5）
