肝胆胰外科杂志2017，Vol.29Issue(2)：134-137,144,5.DOI:10.11952/j.issn.1007-1954.2017.02.011
乌司他丁联合粉防己碱预处理对大鼠肝缺血再灌注损伤的保护作用及机制研究
Protective effect and mechanism of ulinastatin combined with tetrandrine pretreatment on hepatic ischemia-reperfusion injury in rats
摘要
Abstract
Objective To observe the protective effect and to analyze the mechanism of ulinastatin (UTI) combined with tetrandrine (TET) pretreatment of hepatic ischemia-reperfusion injury (HIRI) in rats.MethodsA total of 40 female SD rats were randomly divided into 5 groups, each group 8 rats: Sham operation group (Sham group), hepatic ischemia reperfusion injury group (HIRI group), ulinastatin pretreatment group (UTI group), tet-randrine pretreatment group (TET group), and ulinastatin combined with tetrandrine pretreatment group (UTI+ TET group). Five groups were respectively injected with normal saline (5 mL/kg), normal saline (5 mL/kg), UTI (5 mL/kg), TET (5 mL/kg), and UTI (5 mL/kg) combined with TET (5 mL/kg) by intraperitoneal injection 30 min before ischemia. The serum and liver tissue samples were collected 6 h after hepatic ischemia reperfusion. The activities of lactate dehydrogenase (LDH) and alkaline hosphatase (ALP) in serum were detected. The activities of myeloperoxidase (MPO), glutathione peroxidase (GSH-Px) and the expression level of nuclear transcription fac-tor kappa B (NF-κB) p65 in liver tissue were tested.Results (1)The activities of LDH in group HIRI, UTI, TET and UTI+TET were significantly higher than that in Sham group (P<0.01). The activities of ALP in group HIRI and TET were higher than those in Sham group (P<0.05,P<0.01). The activities of LDH and ALP in group UTI, TET and UTI+TET were significantly lower than those in HIRI group (P<0.01). The activities of LDH and ALP in UTI+TET group were significantly lower than those in group UTI and TET (P<0.01). (2)Compared with Sham group, the activities of MPO were significantly increased, the activities of GSH-Px were significantly decreased in group HIRI, UTI, TET and UTI+TET (P<0.01). In group UTI, TET and UTI+TET, the activities of MPO were significantly lower, and the activities of GSH-Px were significantly higher than that in HIRI group (P<0.01). The activities of MPO were significantly lower, the activities of GSH-Px were significantly higher in UTI+TET group than those in group UTI and TET (P<0.01). (3)The relative expressions of NF-κB p65 in group HIRI, UTI, TET and UTI+TET were higher than those in Sham group (P<0.01). The relative expressions of NF-κB p65 in group UTI, TET and UTI+TET were lower than that in HIRI group (P<0.01). The relative expressions of NF-κB p65 in UTI+TET group were lower than that in group UTI and TET (P<0.01).ConclusionUTI combined with TET has a protective effect on HIRI, the mechanism may be related to inhibiting hepatic lipid peroxidation in hepatic cells, enhancing antioxidant capacity and down regulating the expressions of NF-κB p65 in liver tissue.关键词
乌司他丁/粉防己碱/肝缺血再灌注损伤/大鼠Key words
ulinastatin/tetrandrine/hepatic ischemia-reperfusion injury/rats分类
医药卫生引用本文复制引用
钟毓杰,刘忠民,黄勇平,邱晓俊..乌司他丁联合粉防己碱预处理对大鼠肝缺血再灌注损伤的保护作用及机制研究[J].肝胆胰外科杂志,2017,29(2):134-137,144,5.基金项目
深圳市科技研发资金知识创新计划项目(JCYJ20140414123738261) （JCYJ20140414123738261）
深圳市龙岗区科技计划扶持项目(PT2015094). （PT2015094）
