河南农业大学学报2017，Vol.51Issue(1)：48-52,5.
牛IRS1基因3'UTR的克隆、鉴定及生物信息学分析
Cloning, identification and bioinformatics analysis of the 3'UTR of bovine IRS1
摘要
Abstract
Using bovine mammary tissue as material,bovine IRS1 gene 3'UTR full length was cloned by 3'RACE technology.Measured sequence was further validated.At the same time,TargetScan 7.0 and PicTar sofeware were used to predict the possible binding microRNA.Minimal binding free energy of the multiple software conjunct predicting microRNAs was analyzed.The results showed that the 1327bp 3'UTR full length of bovine IRS1 was successfully cloned.The detected sequence was 99％ consistent with the predicted sequences in NCBI.MiR-128,miR-96 and miR-27a were predicted by two authoritative software together.The minimum free energy of three binding sites was-89.58,-87.91 and-100.88 kJ · mol-1,respectively.These results suggested that the bovine IRS1 gene expression might be regulated by the three microRNAs.关键词
牛/胰岛素受体基质1/3'非翻译区/3'RACE/最小自由能Key words
bovine/IRS1/3'untranslated region/3'RACE/minimum free energy分类
农业科技引用本文复制引用
李文清,李中举,孟志鹏,牛梦宇,李万利..牛IRS1基因3'UTR的克隆、鉴定及生物信息学分析[J].河南农业大学学报,2017,51(1):48-52,5.基金项目
国家自然科学基金项目(31501978) （31501978）
河南省高等学校重点科研项目(16A230010) （16A230010）
