解放军医学杂志2017，Vol.42Issue(2)：128-133,6.DOI:10.11855/j.issn.0577-7402.2017.02.07
西咪替丁对低剂量电离辐射致大鼠氧化应激的保护作用
Protective effects of cimetidine against oxidative stress induced by low-dose irradiation
摘要
Abstract
Objects To study the protective effects of cimetidine against oxidative stress in rats induced by cumulative low-dose irradiation.Methods Sixty SD rats were randomly divided into 6 groups (10 each):normal control group,model control group,lentinan group [89mg/(kg.d)] and 3 dose groups of cimetidine.After oral administration,all the rats were exposed to γ-ray irradiation 8 hours/day for 12 days,and sacrificed on the 13th day.The activities of superoxide dismutase (SOD),glutathione peroxidase (GPx),catalase (CAT) and the content of malondialdehyde (MDA) in serum,liver,thymus and spleen were determined.By using the superoxide anion radical system,hydroxyl radical system,H2O2 radical system,oxidation system of linoleic acid induced by alkane radical system and diphenyl picryl hydrazinyl radical (DPPH) radical system,the antioxidation activities of cimetidine were detected.Results The activities of SOD in liver and thymus decreased significantly,the GPx activity in serum,liver and spleen decreased significantly and MDA level in serum,liver and spleen increased significantly after 0.3Gy cumulative ionizing radiation.Cimetidine enhanced the activities of antioxidant enzymes in serum and organs,and reduced the MDA level.In a certain concentration range,cimetidine had different scavenging effects onto these radical systems,and showed good performance in hydroxyl radical.Conclusion Cimetidine can effectively ameliorate the oxidative stress from low-dose cumulative irradiation by scavenging free radicals,increase the activity of antioxidant enzymes and reduce the content of lipid peroxidation products,thus presents a potential radio protective effect.关键词
西咪替丁/辐射/自由基/抗氧化剂/氧化性应激Key words
cimetidine/radiation, free radicals/antioxidants/oxidative stress分类
医药卫生引用本文复制引用
赵忆宁,何颖,沈先荣,张俊玲,蒋定文,王庆蓉,赵勇,潘迎捷..西咪替丁对低剂量电离辐射致大鼠氧化应激的保护作用[J].解放军医学杂志,2017,42(2):128-133,6.基金项目
国家科技重大专项课题(2004ZX09J14103-07B)This work was supported by the Major Project of National Science and Technology (2004ZX09J14103-07B) （2004ZX09J14103-07B）
