解放军医学杂志2017，Vol.42Issue(2)：134-139,6.DOI:10.11855/j.issn.0577-7402.2017.02.08
褪黑激素对海水浸泡兔脊髓损伤的保护作用
Protective effect of melatonin against spinal cord injury from seawater immersion in rabbits
摘要
Abstract
Objective To observe the protective effects of melatonin against spinal cord injury from seawater immersion in rabbits.Methods The 120 mature and health New Zealand White rabbits,weight range from 2.6 to 2.9kg,were randomly divided into four groups (30 each):control group,ethanol group,melatonin group (100mg/kg),methylPrednisolone group (30mg/kg).The rabbit model of spinal cord injury were built by modified Allen's method taking the 10th thoracic vertebra as a center,seawater immersion for 60 minutes,and then by grouping to give the appropriate treatment.After each group was given the corresponding treatment,six rabbits in each group were randomly selected at 1,6,12,24 and 48 hours five different time points.The neurological function scores of the rabbits were evaluated by Tarlov method,the spinal cord ofT9 to T.which were obtained from all the groups were used for further study,including immunohistochemical detection of apoptosis proteins:Bax,Bcl-2,neurofilament protein 200 (NF200) and in situ end labeling (TUNEL) method to detect spinal neuronal cell apoptosis.Results Within each observation time point,the Tarlov score was higher in melatonin group and methylprednisolone group compared with control group and ethanol group (P＜0.05),there was no significant difference between melatonin group and methylprednisolone group (P＞0.05).The expressions of Bcl-2 and NF200 were significantly higher in melatonin group and methylprednisolone group compared with control group and ethanol group,while Bax expression was significantly lower (P＜0.05).There were no significant difference between melatonin group and methylprednisolone group in the expression of three proteins (P＞0.05).The TUNEL-positive apoptotic cells were fewer in melatonin group and methylprednisolone group compared with control group and ethanol group (P＜0.05),and there was no significant difference between melatonin group and methylprednisolone group (P＞0.05).Conclusion Melatonin has protective effect against spinal cord injury from seawater immersion in rabbits,no difference in efficacy exists compare with methylprednisolone.关键词
脊髓损伤/褪黑激素/甲泼尼龙/海水浸泡/细胞凋亡Key words
spinal cord injuries/melatonin/methylprednisolone/seawater immersion/apoptosis分类
医药卫生引用本文复制引用
王涛,吴成如,王海峰,朱捷,李葛威,洪鹤,杨强,方健..褪黑激素对海水浸泡兔脊髓损伤的保护作用[J].解放军医学杂志,2017,42(2):134-139,6.基金项目
南京军区“十一五”医学科研重点课题(09Z008)This work was supported by the "Eleventh Five-Year Plan" Medical Science Development Foundation of Nanjing Command (09Z008) （09Z008）
