国际眼科杂志2017，Vol.17Issue(4)：619-622,4.DOI:10.3980/j.issn.1672-5123.2017.4.07
外加直流电场对人晶状体上皮细胞移行和增殖活力的影响
Effect of the electric field on the migration and proliferation of human lens epithelial cells
摘要
Abstract
·AIM: To observe the effect of applied direct electric field ( EF) on the migration, cell growth, apoptosis and cell cycle of human lens epithelial cells ( hLECs) . ·METHODS: HLE - B3 cells were exposed to EF at 100mV/mm, 200mV/mm and 400mV/mm, respectively. Cells without EF - exposure were treated as normal controls. Photos of HLE-B3 cells were captured before and after EF - exposure, and the cell numbers were calculated. Apoptosis and cell cycle were detected with flow cytometry after EF-exposure for 24h. ·RESULTS: After exposure to EF at 400mV/mm for 3h, HLE-B3 cells showed directed migration to the cathode. The cell number of HLE-B3 cells decreased gradually with continuous EF-exposure, and decreased by 12. 6%at 6h and by 18.6% at 12h, respectively (P<0.05). After exposure to EF at 100mV/mm, 200mV/mm and 400mV/mm for 24h, the apoptosis rates of HLE - B3 cells increased dramatically compared to that of the normal control, which were (9. 2±1. 9)%, (23. 9±2. 6)% and (54±2. 5)%, respectively (P<0. 05). Accordingly, the results of flow cytometry showed that cell numbers in G2/M phase were increased after EF-exposure, which were (13. 8±2. 2)% and (15. 6±2. 5)% at 200mV/mm and 400mV/mm, respectively (P<0. 05). ·CONCLUSION: Applied direct EF induces directed migration of HLE-B3 cells and inhibits the cell growth with the prolongation of the EF-exposure time. EF also induces cell apoptosis and G2/M phase inhibition of the cell cycle in HLE-B3 cells.关键词
电场/人晶状体上皮细胞/细胞移行/凋亡/细胞周期Key words
electric field/human lens epithelial cell/cell migration/apoptosis/cell circle引用本文复制引用
韩静,刘珺,严宏,闫小龙..外加直流电场对人晶状体上皮细胞移行和增殖活力的影响[J].国际眼科杂志,2017,17(4):619-622,4.基金项目
国家自然科学基金青年项目(No. 81200617) （No. 81200617）
陕西省自然科学基金(No. 2012JM4055)National Natural Science Foundation of China (No. 81200671) （No. 2012JM4055）
Natural Science Foundation of Shaanxi Province (No. 2012JM4055) （No. 2012JM4055）
