国际眼科杂志2017，Vol.17Issue(4)：658-660,3.DOI:10.3980/j.issn.1672-5123.2017.4.17
视网膜微血管内皮细胞的体外分离和培养方法新进展
Progress on separation and cultivation of retinal microvascular endothelial cells in vitro
摘要
Abstract
·There are a variety of ways for isolation and selective cultivation of retinal microvascular endothelial cells in vitro, in which the newly reported tissue culture with collagenase digestion method are widely used for simple, economical, high purity and good activity of obtained endothelial cells. As a result of the limitation of person eye donor, researchers often use rat retinal microvascular endothelial cells that are highly homologous to human beings to experiment in vitro. In this paper, the methods of culturing retinal microvascular endothelial cells in vitro will be elaborated from the aspects of animal species, added factors, cell purification and identification in vitro. At the same time, the advantages and disadvantages of these methods in the process of operation are briefly described, which can provide a reference for the researchers to select suitable cultivation methods.关键词
视网膜微血管内皮细胞/体外培养/进展Key words
retinal microvascular endothelial cells/cultivation in vitro/progress引用本文复制引用
杨敏,罗向霞,王晗,冯玉沛..视网膜微血管内皮细胞的体外分离和培养方法新进展[J].国际眼科杂志,2017,17(4):658-660,3.基金项目
国家自然科学基金项目(No. 81460685) Natural Science Foundation of China ( No. 81460685) （No. 81460685）
